| 周 梦，赵 勇，李珊珊，徐 渴，张树华，杨学举.小麦籽粒清蛋白和球蛋白条件和非条件QTL分析[J].麦类作物学报,2018,(8):883 |
| 小麦籽粒清蛋白和球蛋白条件和非条件QTL分析 |
| Analysis of Conditional and Unconditional QTL for Grain Albumin and Globulin Content in Wheat (Triticum aestivumn L.) |
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| DOI：10.7606/j.issn.1009-1041.2018.08.01 |
| 中文关键词: 小麦 清蛋白 球蛋白 非条件QTL 条件QTL |
| 英文关键词:Wheat Albumin Globulin Unconditional QTL Conditional QTL |
| 基金项目:国家重点研发计划项目（2017YFD0100600）；河北省科技支撑计划项目（16226320D） |
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| 中文摘要: |
| 小麦蛋白含量的提高对营养和加工品质具有重要的意义。本研究在2年3点6个环境下，以小麦品种“花培3号×豫麦57”构建的双单倍体（doubled haploid，DH）群体为材料，通过条件和非条件QTL鉴定与小麦开花后5个时期籽粒清蛋白和球蛋白含量积累有关的基因或数量性状基因座。结果表明，基于完备区间作图法，共检测到40个非条件QTLs和34个条件QTLs，单个位点的贡献率范围为6.44%~25.13%，其中QAlu1B-3在6个环境下的第4和第5时期均被检测出。本研究结果为了解不同灌浆期QTL选择性表达奠定了理论基础，检测到的QTL可能对小麦籽粒品质的提高有重要贡献。 |
| 英文摘要: |
| The improvement of wheat protein-fraction contents is important in wheat breeding for nutritional and processing quality. A set of 168 doubled-haploid lines was used to identify dynamic expression of genes or quantitative trait loci (QTLs) associated with accumulation of different protein-fractions through conditional and unconditional analysis of five post-flowering stages of wheat at three locations over 2 years. The QTLs were analyzed using an inclusive composite interval mapping approach. A total of 40 unconditional QTLs and 34 conditional QTLs for protein-fraction contents were detected and individual QTLs in the six environments explained between 6.44% and 25.13% of observed phenotypic variation.,among which QAlu1B-3 was persistently expressed at 2 stages in six environments. The results provide a theoretical base for understanding the genetic basis of selective expression of QTLs during different grain-filling stages. The detected QTLs may have important contributions to grain quality in wheat. |
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