In order to determine the optimum planting density and the amount of selenium application for selenium-enriched wheat in Xinjiang, three levels of planting density[4.5 million plants·hm-2 (D1), 6 million plants·hm-2 (D2) and 7.5 million plants·hm-2 (D3)], and five concentrations of sodium selenite[0 g·hm-2 (Se1), 15 g·hm-2 (Se2),30 g·hm-2 (Se3), 45 g·hm-2 (Se4) and 60 g·hm-2 (Se5)] were conducted at the early filling stage, respectively. The effects of different treatments on spring wheat yield and its components, selenium content, selenium accumulation, phosphorus and potassium content were analysed. The results showed that spike number, spike number per spike, spike length, thousand-grain weight and yield under D2 treatment reached the maximum, which were 663.00 × 104·hm-2, 45.37, 10.45 cm, 44.38 g and 7 291.38 kg·hm-2, respectively. Of them, the yield was increased by 13.24% and 9.89%, respectively,compared with that of D1 and D2. Selenium application increased the yield of wheat by 4.59%-16.21%. The content of organic selenium in the grains after selenium application reached to 140.90-346.32 μg·kg-1, accounting for 79.17%-82.47% of the total selenium content. The planting density had no significant effect on the total selenium, organic selenium, inorganic selenium content and selenium fortification index of grain, but had a significant effect on the grain selenium accumulation and selenium utilization rate, of which, the accumulation of selenium under D2 treatment was the largest. The interaction of planting density and selenium had a significant effect on the accumulation of selenium, but had no significant effect on other indices. Selenium application significantly increased nitrogen and phosphorus content of grain but decreased potassium content. The content of nitrogen and phosphorus under Se4 were the highest, 4.55% and 16.67% higher than that under Se1, and the content of potassium under Se1 was highest, accounting for 0.28%-0.29%. Under the experimental conditions, the optimum conditions were 6 million plants·hm-2 for planting density and 45 g·hm-2 for the amount of selenium application, respectively. |