In order to provide scientific basis for high water and nitrogen use efficiency in wheat cultivation, using Baomai 10 and Shimai 22, two wheat cultivars suitably planted under the affluent water as well as fertilizer conditions and the water saving conditions, respectively, the effects of nitrogen(N) application level together with reduced irrigation on N acquisition and utilization, at the key growth and development stages were investigated. Under the sufficient-N conditions, the plants exhibited increased N accumulative amounts and accumulation rates, at all growth stages, showing the elevated N accumulative amounts in grain, stem, leaf and glume at maturity stage. the enhanced transportation amounts derived from pre-flowering N, and the increased yields compared with those under the deficient-N conditions. In addition, Baomai 10 and Shimai 22 both displayed similar N accumulative amounts and comparable N accumulation rates at different growth stages, and comparable N utilization associated parameters and yields under the sufficient-N conditions. However, under the deficient-N treatments, compared with Baoming 10, Shimai 22 showed increased N accumulative amounts, enhanced N accumulation rates, elevated N utilization parameters, such as increased N harvest index, transportation amounts of pre-flowering stored N, translocation rates of pre-flowering stored N, and partial productivity of the N fertilizer. Among these N utilization-associated traits, most of them were shown to be significant difference. The results in this study suggest that the cultivars examined possess drastic variation in response to the external N application input. Compared with Baomai 10, Shimai 22 has relatively strong capacity in N acquisition and possesses improved efficiency in using and transferring N from the vegetative tissues to the harvest organ. Our investigation indicates that the drought-tolerant cultivar, such as Shimai 22, has strong capacity of N acquisition and utilization than the drought-sensitive cultivar Baomia 10. which contributes to the relatively high productivity of wheat drought-tolerant cultivar challenged by the drought stress. |