| To explore the main reason of oat grain mildew under autumn rainy conditions, bacteria diversity of oats grain surface and differences between mouldy oat grain and normal oat grain during harvest season were examined. An experiment was carried out to study the diversity of oat grain surface bacteria using high throughput sequencing technology. The results showed that species richness and community diversity of bacteria and fungi on mouldy oat grain surface were significantly higher than those of normal oat grain. From genus classification level, the main bacteria categories identified in mouldy oat grain are Erwinia, Pseudomonas, Klebsiella, Chryseobacterium, Ramlibacter, Agrobacterium, Curtobacterium, Paenibacillus and Pedobacter, taking up 20%, 15%, 6%, 3.7%, 2.6%, 1.9%, 1.6%, 1.6% and 1.3%, respectively. The main bacteria categories identified in normal oat grain are Hymenobacter, Erwinia and Pseudomonas, taking up 2.1%, 1.8% and 1.4%, respectively. The main fungi identified in mouldy oat grain are Alternaria, Pyrenophora, Gibberella, Davidiella, accounting for 38.7%, 25.2%, 7.2% and 2.39%; the main fungi identified in mouldy oat grain are Pyrenophora, Alternaria, Radulidium, Davidiella，Gibberella, and Cryptococcus, accounting for 21.7%, 7.6%, 7.4%, 3.9%, 1.24% and 1.1%, respectively. By comparing the abundance of surface bacteria in two samples and the morphological characteristics of different bacterial populations, it was calculated that mildew of oat grain may be caused by Alternaria. |
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