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作者	单位
周国雁，隆文杰，雷涌涛，陈丹，蔡青，伍少云	（云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业部西南作物基因资源与种质创制重点实验室，云南昆明650205）

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中文摘要:

为研究临沧铁壳麦种子在发芽期的α-淀粉酶活性及其编码基因相对表达量与种子贮藏温度、贮藏时间及生活力间的关系，分别采用3, 5-二硝基水杨酸法和实时定量PCR法测量α-淀粉酶活性及其编码基因的相对表达量。结果表明，与低温贮藏种子相比，高温贮藏种子的发芽期α-淀粉酶活性和 amy1基因的相对表达量均显著升高； amy3基因的相对表达量则显著下降，与α-淀粉酶活性呈极显著正相关；二者与生活力指标（如发芽速率、发芽指数、发芽势、发芽率和活力指数等）呈显著或极显著正相关；而低温贮藏种子的发芽期α-淀粉酶活性与 amy3基因的相对表达量呈显著或极显著负相关。说明贮藏温度尤其是高温不仅强烈影响种子发芽期α-淀粉酶活性及其基因相对表达量，而且可明显改变二者与生活力指标间的相关性。

英文摘要:

In order to study the relationships among α-amylase activity, relative expression quantitation(REQ) of their coding genes, storage temperature, storage time and seed vitality indices of Lincang hulled wheat(Triticum aestivum ssp. yunnanense) at germination stage, the methods of 3, 5-Dinitrosalicylic acid(3, 5-DNSA) and real time polymerase chain reaction(RT-PCR) were employed to measure α-amylase activity and REQ of their coding genes, respectively. The results showed that compared with storage at low temperature, the α-amylase activity and REQ of amy1 gene in seeds stored at high temperature were significantly increased. However, REQ of amy3 gene was obviously decreased and had a significant positive correlation with α-amylase activity, and both REQ of amy3 gene and α-amylase activity had a significant positive correlation with seeds viability indices, such as germination rate, germination index, germination potential, germination rate and vigor index. There was a significant negative correlation between α-amylase activity and REQ of amy3 gene in seeds stored at low temperatures. The results mainly showed that seed storage temperature not only strongly affected α-amylase activity and REQ of its coding gene, but also greatly altered correlation with seed viability indices.

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