丁梦云,刘天相,孙宇慧,柴乖强,高 欣,王 军,王中华,李春莲.小麦QTL富集区 QC-4BS重要农艺性状基因的精细定位及 Rht1基因的效应分析[J].麦类作物学报,2018,(3):262 |
小麦QTL富集区 QC-4BS重要农艺性状基因的精细定位及 Rht1基因的效应分析 |
Fine Mapping of Important Agronomic Traits of a QTL Cluster QC-4BS and Effect Analysis of Rht1 in Wheat(Triticum aestivum L.) |
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DOI:10.7606/j.issn.1009-1041.2018.03.02 |
中文关键词: 小麦 基因定位 QTL富集区 Rht1 基因 |
英文关键词:Wheat Gene mapping QTL cluster Rht1 gene |
基金项目:陕西省自然科学基础研究计划项目(2017ZDJC-10);陕西省重点科技创新团队项目(2014KCT-25) |
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中文摘要: |
为了探明位于小麦4BS染色体上的一个QTL富集区 QC-4BS重要农艺性状的遗传效应,对 QC-4BS重要农艺性状基因进行了精细定位,并分析了位于 QC-4BS内的 Rht1基因对小麦株高(PH)、穗粒数(KNPS)、千粒重(TKW)、蛋白质含量(GPC)及容重(TW)等性状的遗传效应。结果表明, QC-4BS主要位于小麦4BS染色体的 IWA1846至IWA4662标记区间,长度为11.9 cM,对应的物理距离约为15.17 Mb,其中控制PH的QTL为 Rht1基因,距离IWA1846标记约7.16 Mb;控制GPC和KNPS的位点接近IWA482标记;控制TW的位点接近基因 Rht1。 Rht1基因除了具有明显的降低小麦PH的效应外,也可影响小麦的某些产量及品质性状,具体表现为增加小麦KNPS,降低TKW、GPC及TW。 |
英文摘要: |
To fully understand the genetic effect of QC-4BS,a major QTL cluster on chromosome 4BS associated with important agronomic traits of wheat(Triticum aestivum L.),fine mapping of QC-4BS was conducted and the effect of dwarf gene Rht1 on wheat important traits including plant height(PH),kernel number per spike(KNPS),thousand kernel weight(TKW),grain protein content(GPC),and test weight(TW) were analysed. The results showed that QC-4BS was located at the marker interval of IWA1846-IWA4662 on chromosome 4BS with the genetic distance of 11.96 cM corresponding to the physical distance of 15.17 Mb. In QC-4BS, Rht1 is responsible for PH QTL and positioned about 7.16 Mb from the marker IWA1846; GPC and KNPS loci are close to the marker IWA482; and TW locus is near to Rht1. The dwarf gene Rht1 has significant effect on not only PH but also KNPS,TKW,GPC and TW. Rht1 could decrease the phenotypic value of PH,TKW,GPC and TW,but increase the value of KNPS in wheat. |
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