| 高新菊,郭秀玲,陈 威,包来仓,马毅辉,秦光宇,祖均怀,王恒亮.河南省麦田猪殃殃对苯磺隆的抗性及ALS基因突变研究[J].麦类作物学报,2017,(11):1518 |
| 河南省麦田猪殃殃对苯磺隆的抗性及ALS基因突变研究 |
| Resistance to Tribenuron-Methyl and ALS Mutations in Galium aparine in Wheat Fields in Henan Province |
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| DOI：10.7606/j.issn.1009-1041.2017.11.16 |
| 中文关键词: 河南省 猪殃殃 苯磺隆 抗性 ALS基因突变 |
| 英文关键词:Henan province Galium aparine Tribenuron-methyl Resistance ALS mutation |
| 基金项目:河南省农业科学院自主创新专项(2013ZZ22,2016ZC43) |
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| 中文摘要: |
| 为明确河南省部分地区麦田猪殃殃对苯磺隆的抗性水平及抗性靶标分子机制，对18个猪殃殃种群进行了乙酰乳酸合成酶（acetolactate synthase，ALS）离体活性测定和ALS基因突变分析。结果表明，种群PDS-1、ZK-1、LH-1对苯磺隆产生了较高的抗性，ALS离体活性测定所得I50分别为11.27、10.26、8.19 μmol·L-1，抗性指数分别为93.92、85.50、68.25。种群ZK-1的6个检测株中，ALS基因第590位的C突变为T，ALS酶第197位脯氨酸（CCC）突变为亮氨酸（CTC）；种群PDS-1的6个检测株中，ALS基因第1 128位的T突变为A，ALS酶第376位天冬氨酸（GAT）全部突变为谷氨酸（GAA）；种群LH-1的6个检测株中，3株未检测到突变，另外3株的ALS基因第1 128位的T突变为G，ALS酶第376位天冬氨酸（GAT）突变为谷氨酸（GAG）。靶标ALS基因突变可能是猪殃殃对苯磺隆产生高抗性的重要原因之一。 |
| 英文摘要: |
| To investigate the resistance levels and molecular mechanism of Galium aparine to tribenuron-methyl in winter wheat fields in Henan province,eighteen Galium aparine populations were assessed by acetolactate synthase(ALS) activity in vitro assay and analysis of ALS mutations.The results showed that Galium aparine populations PDS-1,ZK-1,and LH-1 had higher levels of resistance than those of others,and their I50 of ALS activity in vitro assay were 11.27,10.26 and 8.19 μmol·L-1,respectively,and the corresponding relative resistance index were 93.92,85.50 and 68.25,respectively.ALS sequences analysis indicated that C→T at 590th base proline(CCC) at position 197 of the ALS was substituted by leucine(CTC) in six PDS-1 plants.Aspartic acid(GAT) at position 376 was substituted by glutamic acid(GAA) in six ZK-1 plants.Aspartic acid(GAT) at position 376 was substituted by glutamic acid(GAT) in three LH-1 plants.The Pro197 or Asp376 mutations in ALS could be one of important reasons for the observed high resistance to tribenuron-methyl. |
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