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谢 燕,张庆龙,胡 玲,王法宏,李豪圣,孔令安.PEG胁迫对不同小麦品种幼苗抗旱生理指标的影响[J].麦类作物学报,2017,(7):947
PEG胁迫对不同小麦品种幼苗抗旱生理指标的影响
Physiological Responses of Different Wheat Cultivars to PEG Stress
  
DOI:10.7606/j.issn.1009-1041.2017.07.12
中文关键词:  PEG胁迫  冬小麦  根系形态特征  叶绿素荧光参数
英文关键词:PEG stress  Wheat  Root morphology  Chlorophyll fluorescence parameters
基金项目:山东省现代农业产业技术体系项目(SDAIT-01-06);国家小麦现代农业产业技术体系项目(CARS-3-1-21);山东省自然科学基金项目(ZR2016CM39);国家公益性行业(农业)科研专项(201303109-07)
作者单位
谢 燕,张庆龙,胡 玲,王法宏,李豪圣,孔令安 (山东省农业科学院作物研究所山东济南 250100) 
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中文摘要:
      为给小麦抗旱育种的早期筛选工作提供理论依据,以济麦262(抗旱型)、烟农19(中间型)和临麦2号(水分敏感型)为材料,采用PEG模拟干旱胁迫,探究干旱胁迫对不同小麦品种幼苗生物量、含水量、根系形态特征、抗氧化酶活性和叶绿素荧光参数的影响。结果表明,PEG胁迫下,三个品种中济麦262的生物量最大,地下部含水量显著高于临麦2号和烟农19;其根系的总长、总投影面积、总面积和总体积最大,且受抑制程度最低;其抗氧化酶活性在一定程度上明显增强,尤其是叶片和根系过氧化物酶、根系超氧化物歧化酶及叶片过氧化氢酶活性增强幅度显著高于临麦2号和烟农19;其叶片光化学荧光淬灭系数(qP)和PSⅡ的实际光化学效率与CK相比均呈下降的趋势,其中qP下降程度最低,荧光非光化学淬灭显著增强且高于其他两个品种。综上所述,在PEG胁迫下抗旱品种的生长受抑制程度最小,清除氧自由基能力较强,光能利用率高。
英文摘要:
      In order to provide theoretical basis for drought resistance wheat breeding,wheat varieties,Linmai 2,Yannong 19 and Jimai 262,were hydroponically cultivated in Hoagland solution(control) or solution containing 25% PEG(water stress) to investigate the effects of water stress on plant biomass,water content,root morphology,antioxidant enzyme activity and chlorophyll fluorescence parameters in seedlings. The results showed that under water stress,Jimai 262 showed the largest biomass among the three wheat varieties,and its root water content was significantly higher than that of Linmai 2 and Yannong 19. Root assessment using a WinRHIZO scanner showed that the total root length,total projected area,total area and total volume under PEG treatment were less affected in Jimai 262 compared with Linmai 2 and Yannong 19. Activity of antioxidant enzymes of Jimai 262 was greatly increased,especially the activities of POD,SOD in roots and CAT in leaves were significantly increased,compared to that of Linmai 2 and Yannong 19. Compared with control,both qP and ΦPSⅡ in leaves of examined cultivars were decreased and qP of Jimai 262 had a slight reduction. NPQ of Jimai 262 was significantly increased under water stress,compared with control,and higher than that of the other two varieties. In conclusion,under PEG treatment,Jimai 262 showed the smallest degree of inhibition on growth,stronger ability of scavenging oxygen free radical,higher light utilization efficiency and higher resistance to water stress,compared to Linmai 2 and Yannong 19.
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