许 峰,李文阳,闫素辉,张从宇,郑甲成,杜军丽,张子学,时侠清.小麦抗赤霉病主效QTL的聚合效应分析[J].麦类作物学报,2017,(5):585 |
小麦抗赤霉病主效QTL的聚合效应分析 |
Analysis of Pyramiding Effect of Major QTLs for Resistance to Scab in Wheat |
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DOI:10.7606/j.issn.1009-1041.2017.05.03 |
中文关键词: 小麦 抗赤霉病 QTL 聚合效应 |
英文关键词:Wheat Scab resistance QTL Pyramiding effect |
基金项目:安徽省教育厅自然科学基金重点项目(KJ2015A221);安徽科技学院重点学科资助项目(AKZDXK2015A03) |
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中文摘要: |
为了明确小麦抗赤霉病主效QTL的抗病效应,基于分子标记辅助选择,将来源于抗病品种苏麦3号的 Fhb1、 Fhb2和望水白的 Fhb4、 Fhb5回交导入感病品种矮抗58中,构建出30个不同QTL组合的株系,分别采用单花滴注和喷洒孢子悬浮液接种法,对其开展抗侵入、抗扩展和综合抗性鉴定。结果表明,携带 Fhb1和 Fhb2的株系表现出显著的抗扩展能力, Fhb1的效应显著高于 Fhb2,但二者都没有抗侵入效应;携带 Fhb4和 Fhb5的株系表现出明显的抗侵入性,二者累加后效应更显著,但均没有明显的抗扩展能力;不同抗性类型的QTL聚合后,其综合抗病性比单个QTL更显著。说明在育种中,将不同抗性类型的QTL聚合后比单个QTL的抗赤霉病效果更佳。 |
英文摘要: |
In order to clarify the wheat scab resistance effect of major QTLs,four major QTLs were backcrossed into susceptible variety Aikang 58 based on molecular marker assisted selection,and 30 different combinations of QTL were conducted,which were used for inoculation,resistance to expansion and comprehensive resistance identification by single floret inoculation and spraying spores inoculation,respectively. Among them, Fhb1 and Fhb2 are derived from Sumai 3,and Fhb4 and Fhb5 are originated from Wangshuibai. The results showed that the lines containing Fhb1 and Fhb2 performed significant resistance to expansion,and the effect of Fhb1 was significantly higher than that of Fhb2,but neither of them had anti-penetration effect. The lines with Fhb4 and Fhb5 presented obvious resistance to penetration,and the cumulative effect of them was more remarkable than that of individual Fhb4 or Fhb5,but there was no obvious ability to anti-expansion effect. The lines with different resistance types of QTLs had more comprehensive resistance than those with any single resistance type of QTL. Therefore,pyramiding different resistance types of QTLs is more effective than single QTL for scab resistance in wheat breeding practice. |
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