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武炳瑾,冯 洁,崔紫霞,张传量,孙道杰.利用90K基因芯片进行小麦株高QTL分析[J].麦类作物学报,2017,(5):578
利用90K基因芯片进行小麦株高QTL分析
QTL Analysis of Plant Height by Using 90K Chip Technology
  
DOI:10.7606/j.issn.1009-1041.2017.05.02
中文关键词:  小麦  90K基因芯片  QTL定位  株高
英文关键词:Wheat  90K gene chip  QTL mapping  Plant height
基金项目:国家重点基础研究发展计划(973计划)项目(2014CB138100);陕西省重点科技创新团队项目(2014KCT-25)
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武炳瑾,冯 洁,崔紫霞,张传量,孙道杰 (西北农林科技大学农学院陕西杨凌 712100) 
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中文摘要:
      为给小麦株高标记辅助选择提供可供选择的分子标记,并进一步对株高QTL进行精细定位及相关基因克隆,以小麦骨干亲本周8425B和小偃81衍生的包含102个家系的RIL群体(F8)为材料,利用90K芯片标记构建高密度遗传图谱,在3个环境下对株高进行QTL检测。结果表明,所构建的图谱含有9 290个SNP标记,覆盖了小麦21条染色体的63个连锁群,图谱总长3 894.64 cM,平均标记密度为0.42 cM。共检测到9个控制株高的QTL,分布于1B、4A、4D、6B、7A、7B和7D染色体上,变异解释率为2.23%~ 16.25% 。 QPh.nafu.4D QPh.nafu.4A QPh.nafu.1B-2与前人定位到的位置相同或相近。 QPh.nafu.7A具有较大的LOD值(8.17)和变异解释率(14.69%),为主效QTL。 QPh.nafu.6B QPh.nafu.7B-1 QPh.nafu.7B-2均能在多个环境下使用多种QTL检测方法定位到,可能为新的较稳定的控制株高的QTL。
英文摘要:
      Molecular marker assisted breeding of wheat provides a reference for fine mapping and cloning of plant height genes. In the present study,a population of 102 recombinant inbred lines (F8) was derived from a cross between the elite parents Zhou 8425B and Xiaoyan 81. The population was used to identify quantitative trait loci (QTL) in three environments. A high density genetic map of wheat was constructed by using 90K chip. The constructed genetic linkage map contains 9 290 SNP markers,with a total length of 3 894.64 cM,containing 63 linkage groups and covering the 21 chromosomes of wheat,with an average marker density of 0.42 cM. A total of nine QTLs controlling plant height were found on 1B,4A,4D,6B,7A,7B and 7D chromosomes. The amount of phenotype variation explained (PVE) by individual QTL ranged from 2.23% to 16.25%. QPh.nafu.4D QPh.nafu.4A and QPh.nafu.1B-2 have the same or similar position with the findings in previous studies. QPh.nafu.7A have a high value of LOD (8.17) and PVE (14.69%),which may become a main effect QTL. QPh.nafu.6B, QPh.nafu.7B-1 and QPh.nafu.7B-2 can be detected in multi-environments and methods,which may become the new stable QTLs controlling plant height.
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