| 刘 洋，赵继新，谈宏斌，王秀娟，李家创，梁邦平，刁慧珊，白宇皓，武 军，陈新宏.小麦-滨麦2D/ 2Ns异代换系DM2411的分子细胞遗传学鉴定[J].麦类作物学报,2017,(5):571 |
| 小麦-滨麦2D/ 2Ns异代换系DM2411的分子细胞遗传学鉴定 |
| Molecular Cytogenetic and Morphological Identification of a Wheat-Leymus mollis 2Ns (2D) Substitution Line DM2411 |
| |
| DOI：10.7606/j.issn.1009-1041.2017.05.01 |
| 中文关键词: 小麦 滨麦 代换系 GISH EST-STS SSR |
| 英文关键词:Wheat L.mollis Substitution line GISH EST-STS SSR |
| 基金项目:陕西省自然科学基金项目（2015JM3095）；西北农林科技大学唐仲英育种基金项目 |
|
| 摘要点击次数: 980 |
| 全文下载次数: 577 |
| 中文摘要: |
| 为进一步挖掘利用滨麦优异基因，并丰富小麦遗传种质资源，利用形态学、细胞遗传学、基因组原位杂交（Genomic in situ hybridization，GISH）、EST-STS分子标记、SSR分子标记等技术，对从八倍体小滨麦M842-16和硬粒小麦D4286杂交F7代材料中筛选出的1个遗传稳定的小滨麦异代换系DM2411进行了鉴定。细胞遗传学观察表明，DM2411的染色体主要构型为2n=42=21Ⅱ，遗传稳定。根尖体细胞和花粉母细胞的原位杂交研究表明，DM2411含有1对滨麦Ns基因组。SSR分析表明，DM2411可能缺失了小麦2D染色体。EST分析表明，DM2411可能含有滨麦2Ns染色体。形态学调查表明，DM2411的株高极显著降低。 |
| 英文摘要: |
| A wheat-L.mollis alien substitution line DM2411 was identified from the F7 progenies of octoploid Tritileymus M842-16 and T.durum D4286 by using morphology,cytogenetics,genomic in situ hybridization (GISH),expressed sequence tag-sequence tagged site(EST-STS) and simple sequence repeat (SSR). Cytological studies demonstrated that DM2411 had a chromosome karyotype of 2n=42=21Ⅱ.GISH analysis indicated that DM2411 had a pair of Ns chromosomes from L.mollis. Analysis with SSR and EST-STS markers showed that the wheat 2Ds chromosomes were substituted by a pair of 2Ns chromosomes from L.mollis in DM2411. Agronomic trait evaluations showed that the plant height of DM2411 was dramatically lowered. Therefore,the substitution line DM2411 was a dwarf material,which could be exploited as an intermediate material in wheat breeding programs and genetic resources. |
|
查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
基本信息
