| 胡喜贵,伍碧华.伊斯帕汗小麦 NAM-B1基因序列与蛋白质含量变异的分析[J].麦类作物学报,2017,(3):295 |
| 伊斯帕汗小麦 NAM-B1基因序列与蛋白质含量变异的分析 |
| Variations of NAM-B1 Gene and Grain Protein Content in Triticum ispahanicum Heslot |
| |
| DOI：10.7606/j.issn.1009-1041.2017.03.02 |
| 中文关键词: 伊斯帕汗小麦 NAM-B1基因 籽粒蛋白质含量 |
| 英文关键词:Triticum ispahanicum NAM-B1 gene Grain protein content |
| 基金项目:教育部高等学校博士学科点专项科研基金（博导类）项目(20125103110003)；四川省科技厅国际合作项目（2015HH0021）；河南科技学院引进高层次人才科研启动项目(201010613001) |
|
| 摘要点击次数: 1146 |
| 全文下载次数: 682 |
| 中文摘要: |
| 野生二粒小麦(Triticum dicoccoides Krn.,AABB,2n=4x=28） NAM-B1基因的表达可以加速植株衰老，促进叶片营养向籽粒转移，提高籽粒蛋白质、铁、锌等物质含量。本研究采用同源克隆方法从6份伊斯帕汗小麦（T.ispahanicum Heslot,AABB,2n=4x=28）中克隆得到5个 NAM-B1基因。它们具有典型的NAM基因核苷酸结构特点，均含有3个外显子和2个内含子。与已报道的 NAM-B1基因比对分析发现，居群PI330548中的 NAM-B1基因因核苷酸序列上第11位点T的插入而引起移码突变，具有无功能型基因的结构特征，其他4个则具有功能类型基因的结构特征。4个功能类型 NAM-B1基因的推导氨基酸序列一致性高达99.7%，它们之间仅有5个氨基酸的变异。供试的6份伊斯帕汗小麦之间籽粒蛋白质含量（GPC）存在显著差异。与高GPC材料PI346782相比，低GPC材料PI572904的 NAM-B1基因编码氨基酸序列中存在2个位点的变异，分别为NAC域内的C亚区第88位Q→R和TAR区域第364位P→R的替换。推测伊斯帕汗小麦GPC的变异与其 NAM-B1基因的这些突变存在一定关系。 |
| 英文摘要: |
| The NAM-B1 gene in T.dicoccoides is able to accelerate senescence and increase nutrient remobilization from leaves to developing grains,resulting in improving the grain protein content(GPC) and iron and zinc contents. In this study,five NAM-B1 genes were cloned from six T.ispahanicum accessions using homologous cloning method,which have typical NAM gene nucleotide structure characteristics with three exons and two introns. Compared with previously reported NAM-B1 gene,the base T insertion at the position 11 occurred in the NAM-B1 gene of PI330548,which lead to frame shift mutation,implying it being non-functional while others functional. The deduced amino acid sequences of the four functional NAM-B1 genes had a high identity for 99.7% with only five amino acids variation. The grain protein contents(GPCs) were significantly different among the six tested accessions. In comparison with amino acid sequence of accession PI346782 with high GPC,that of PI572904 with low GPC existed two substitutions of amino acid including the Q→R at locus 88 in sub-domain of NAC domain and the P→R at locus 364 of TAR region,although they all had functional NAM-B1 gene. The results implied that the mutations of NAM-B1 gene may be associated with GPC variation of T.ispahanicum. |
|
查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
基本信息
