The winter wheat cultivars of Tianxuan lines were important commercial wheat varieties in Longnan region,which had better resistance to stripe rust,and higher yield than other wheat varieties since 1950s,and they also played an important role in sustainable controlling of stripe rust in this region. Thirteen winter wheat cultivars,including winter wheat varieties Tianxuan 43-Tianxuan 55,were used as materials,which were commercial wheat varieties in Longnan region,Gansu Province,China since 1990s. Twenty-six single spore isolates of Pst with different spectra of pathogenicity were artificially inoculated at seedling stage in the greenhouse.Combined with pedigree,the results showed that the resistant spectra of Tianxuan 44,Tianxuan 46 and Tianxuan 50 were the same as that of Lovrin 13,maybe possessed Yr9 and unknown genes.Tianxuan 43,Tianxuan 48 and Tianxuan 51,had the same resistant spectra as Moro,maybe possessing Yr10+YrMor. Tianxuan 52,Tianxuan 53 and Tianxuan 55 had the same resistant spectra as Line R55,maybe possessing Yr26.The resistant spectra tested in other wheat varieties were different from the known gene varieties,maybe consisting of unknown resistant gene(s).Nine wheat varieties including Tianxuan 43,Tianxuan 44,Tianxuan 46,Tianxuan 48,Tianxuan 50,Tianxuan 51,Tianxuan 52,Tianxuan 53 and Tianxuan 55 were susceptible to new isolates G22-9 and G22-14 of Pst,but resistant to other races/isolates at all-stage. Two wheat varieties,Tianxuan 47 and Tianxuan 49,were susceptible to CYr33,G22-9 and G22-14 of Pst,but resistant to other races/isolates at all-stage. Two wheat varieties,Tianxuan 45 and Tianxuan 54 were resistant to all tested isolates of Pst. These wheat varieties were planted at seven different locations in Gansu province in 2014 to evaluate their resistance to stripe rust by natural races/isolates of Pst,and the results showed that Tianxuan 44,Tianxuan 45 and Tianxuan 54 were resistant in field,but other ten wheat varieties were susceptible. |