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吕学莲,白海波,董建力,惠 建,孙亚宁,蔡正云,李树华.春小麦旗叶大小相关性状的QTL定位分析[J].麦类作物学报,2016,36(12):1587
春小麦旗叶大小相关性状的QTL定位分析
QTL Mapping for Size Traits of Flag Leaf in Spring Wheat
  
DOI:10.7606/j.issn.1009-1041.2016.12.05
中文关键词:  春小麦  旗叶大小  QTL  RIL群体
英文关键词:Spring wheat  Flag leaf size  Quantitative trait loci  Recombinant inbred line population
基金项目:宁夏回族自治区农业育种专项(2013NYYZ02);宁夏农林科学院科技创新先导资金项目(NKYJ-14-22);国家自然科学基金项目(31160285)
作者单位
吕学莲,白海波,董建力,惠 建,孙亚宁,蔡正云,李树华 (1.宁夏农林科学院农业生物技术研究中心宁夏银川 750002 2.宁夏大学农学院宁夏银川 750021) 
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中文摘要:
      为了发掘控制春小麦旗叶大小相关性状的QTL,以宁春4号/Drasdale构建的包含148个家系的RIL群体F8代为试验材料,在正常灌溉和不同干旱胁迫共3种处理下对小麦开花期旗叶长、宽、面积和周长进行了QTL定位。结果表明,共检测到相关加性QTL 22个,其中,控制旗叶面积的QTL有3个,控制旗叶长的QTL有5个,控制旗叶宽的QTL有9个,控制旗叶周长的QTL有5个,分布于1B、2D、3B、4B、7A、7B和7D染色体上,单个QTL的贡献率在6.58%~39.83%之间。其中, qFLW-2D-2.T1在T1和T3处理下均能够检测到, qFLW-7D.T1在T1和T2处理下也均能够检测到,说明这2个QTL表达不依赖于水分条件,属于稳定表达的QTL。其他QTL只在一种处理下能够检测到,说明其表达依赖于水分条件。同时,本研究也检测到了在不同处理下同时控制2种或3种性状的QTL,推测这些区域的QTL可能为一因多效QTL,但有待于进一步验证。
英文摘要:
      In order to detect the QTLs that control flag leaf size traits in wheat,a population of 148 F8 RILs derived from a cross between Ningchun 4 and Drasdale was used to detect the QTLs for flag leaf area,flag leaf length,flag leaf width and flag leaf perimeter under three different water treatments. The results showed a total of 22 additive-effect QTLs are detected,including three QTLs for flag leaf area,five QTLs for flag leaf length,nine QTLs for flag leaf width,and five QTLs for flag leaf perimeter. These QTLs are located on chromosomes 1B,2D,3B,4B,7A,7B and 7D,with single QTL explaining 6.58% to 39.83% of the phenotypic variances. qFLW-2D-2.T1 was detected under T1 and T3 water treatments,and qFLW-7D.T1 was detected under T1 and T2 water treatments,indicating the expression of these two QTLs were stable and independent of treatments. Other QTLs were detected only in single treatment,thus were treatment-dependent. Some QTLs control two or three traits over-lapped,and these QTLs might be linked to each other or pleiotropic,which needs to be further validated.
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