| The quality characteristics of wheat directly affect its processing and consumption. To explore the wheat quality related QTL (protein,starch,gluten and Zeleny),two different wheat varieties Xinong 981 and Shaanmai 159 were used to develop 169 F2 population and F2:3 lines. The F2 population was exploited to construct genetic map and the F2:3 lines were grown in two different environments for phenotyping. The analysis of variance revealed highly significant difference for protein in two environments,whereas,the quality of starch,gluten and Zeleny were highly significant at Yangling and significant at Sanyuan. 612 pairs of SRAP primers and 1 550 pairs of SSR primers were used to detect polymorphism between parents and F2 population. Of them,66 SRAP and 63 SSR primers showed polymorphism among F2 population. The genetic map and QTL detection results indicated 14 linkage groups including A,B and D genomes with 115 molecular markers constructed,and the whole genetic linkage covered 3 203.67 cM and most markers located on A and B genomes. The 2B chromosome contained maximum number of 21 markers while 1D chromosome revealed minimum three markers. Thirty-three QTLs for quality related traits were identified,including 11 QTLs for protein content,which were distributed on the 1A,3A,5A,6A,2B and 4B chromosomes. Seven QTLs for starch content were distributed on the 1A,6A,4B and 2D chromosomes. Twelve QTLs for gluten content were distributed on the 1A,3A,5A,6A,2B,3B and 4B chromosomes and three QTLs for Zeleny sedimentation value were distributed on the 3A,1B and 3B chromosomes. They could explain 0.69%-2.48%,2.94%-6.99%,0.58%-2.37% and 2.72%-11.31% phenotypic effects,respectively. This study could not detect quality related QTL stable in two environments but detect some QTLs controlling protein,starch and wet gluten traits stable in the same region of chromosome. |
基本信息
