| 蔡 华，赵维萍，葛 奇，阮在浩. Kr1基因在小麦×玉米远缘杂交中的表达及甲基化变异[J].麦类作物学报,2016,36(7):825 |
| Kr1基因在小麦×玉米远缘杂交中的表达及甲基化变异 |
| Expression of Kr1 Gene and Its Methylation Variation in Wheat × Maize Distant Hybridization |
| |
| DOI：10.7606/j.issn.1009-1041.2016.07.01 |
| 中文关键词: 小麦 Kr1基因 远缘杂交 DNA甲基化 |
| 英文关键词:Wheat Kr1 gene Distant hybridization DNA methylation |
| 基金项目:安徽省自然科学基金项目（1308085MC34）；国家公益性行业（农业）科研专项（201403039）；国家重点实验室开放基金项目（ZW2010002）。 |
|
| 摘要点击次数: 1381 |
| 全文下载次数: 760 |
| 中文摘要: |
| Kr1基因是小麦远缘杂交不亲和主效基因，为了解其表达及调控机制，本研究系统比较了小麦自花授粉和小麦×玉米远缘杂交两个过程中 Kr1基因的表达差异，同时分析了这两个过程中 Kr1基因部分序列的DNA甲基化状态。结果表明， Kr1基因在小麦自花授粉过程中始终处于低量表达或不表达状态，而在小麦×玉米远缘杂交过程中的表达呈动态变化，具体表现为授粉前低量表达，授粉后迅速大量表达，24 h后又恢复为低量表达，高峰表达时期在外源花粉授入后0.5～2 h左右。DNA甲基化分析显示，小麦自花授粉前后 Kr1基因一直处于较高水平的甲基化状态，分别为58%和62%；而在授以玉米花粉后， Kr1基因迅速去甲基化，在0.5 h内降到12%，在其后的1、2、24 h内一直维持在10%～12%的低甲基化水平，表明DNA甲基化修饰参与了 Kr1基因在小麦×玉米远缘杂交中的表达调控。 |
| 英文摘要: |
| Kr1 gene is the major gene regulating incompatibility in wheat distant hybridization. To understand its expression and regulation mechanism, the difference of Kr1 expression in the two systems of wheat self-pollination and wheat × maize distant hybridization were compared systematically in this work, and the variations of DNA methylation of Kr1 gene during the self-pollination and distant hybridization processes were analyzed. The results showed that the expression level of Kr1 gene was always low or even no expression in the process of wheat self-pollination. However, its expression showed a dynamic change in wheat × maize distant hybridization, with low expression before pollination, a rapid great increase of expression after pollination, and low expression after 24 h. The peak expression period was present at about 0.5-2 h after pollinated with the foreign pollen. DNA methylation analysis shows that Kr1 gene stays a high level status before and after the self-pollination, with the proportion of 58% and 62%, respectively. However, after pollinated with maize pollen, Kr1 gene is rapidly de-methylated, dropping to a level of 12% in 0.5 h, and maintains at the low level of 10% to 12% methylation in the subsequent 1, 2, and 24 h. These findings indicate that DNA methylation modification is participated in the expression and regulation of Kr1 gene in wheat × maize distant hybridization. |
|
查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
基本信息
