| 王 炜，杨随庄，叶春雷，陈 琛，欧巧明，王红梅，罗俊杰.小麦体细胞无性系HMW-GS组成、蛋白质和赖氨酸含量及SSR位点变异分析[J].麦类作物学报,2016,36(2):157 |
| 小麦体细胞无性系HMW-GS组成、蛋白质和赖氨酸含量及SSR位点变异分析 |
| Analysis of HMW-GS Composition,Protein Content, Lysine Content and SSR Allelic Variations in Wheat Somaclonal Lines |
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| DOI：10.7606/j.issn.1009-1041.2016.02.05 |
| 中文关键词: 小麦 体细胞无性系 变异 品质育种 |
| 英文关键词:Wheat Somaclonal line Variation Quality breeding |
| 基金项目:甘肃省自然科学基金项目（096RJZA027）；甘肃省农科院农业科技创新基金项目（2013GAAS27）；甘肃省农业生物技术研究与应用开发项目（GNSW-2014-15）；甘肃省农科院科技创新工程学科团队项目（2014GAAS06） |
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| 中文摘要: |
| 为明确小麦体细胞无性系后代材料的蛋白质和赖氨酸含量、HMW-GS及SSR位点变异情况，对宁春4号、陇春21号和花培9355三个小麦品种的无性系R4和R5代材料的蛋白质和赖氨酸含量、HMW-GS组成及SSR位点进行检测和鉴定，并分析其变异情况。结果表明，三个小麦品种无性系后代中，编码HMW-GS的三个位点均可发生变异，不同基因型材料的无性系后代的变异频率和位点并不相同，同一基因型无性系后代的不同编码位点的变异频率也不相同；蛋白质和赖氨酸含量出现超亲变异，其中大部分材料在R4代中表现出的变异性状能够稳定传递到R5代中。小麦体细胞无性系SSR位点的变异具有基因型依赖性，蛋白质和赖氨酸含量的变异频率与SSR位点变异频率无直接的相关性。 |
| 英文摘要: |
| In order to clarify the variations in HMW-GS subunit composition,SSR loci,and protein and lysine content in the wheat lines derived from somaclonal variation,the HMW-GS subunit composition,protein content,lysine content and SSR loci in R4 and R5 somaclonal progenies of three genotypes Ningchun 4,Longchun 21 and Huapei 9355 were determined and detected. The results indicated there were variations at all the three loci encoding HMW-GS; the variation frequency and locus in somaclonal lines of different genotypes was not identical; the variation frequency of different loci in somaclonal lines with the same genotype was also different; ultra-parent variations of protein and lysine content were found in somaclonal lines and the traits in most of the R4 generation lines could be passed on to the R5 generation; the variation of SSR locus in somaclonal lines was genotype-dependent; and there was no direct correlation between the variation frequency of protein content,lysine content and that of SSR locus in somaclonal lines. The results will further provide scientific basis for creating germplasm resource and quality breeding in wheat by taking advantage of somaclonal variation. |
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