| 郑青焕，李晓萍，郭 超，昝 凯，陈真真，白宇浩，刘 洋，赵继新，武 军，王中华，陈新宏.21份印度小麦高分子谷蛋白亚基、醇溶蛋白及品质分析[J].麦类作物学报,2016,36(1):62 |
| 21份印度小麦高分子谷蛋白亚基、醇溶蛋白及品质分析 |
| Identification of HMW-GS, Gliadin and Quality Property of Twenty-one Indian Wheats |
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| DOI：10.7606/j.issn.1009-1041.2016.01.09 |
| 中文关键词: 小麦 印度 高分子量谷蛋白亚基 醇溶蛋白 遗传多样性 |
| 英文关键词:Wheat India HMW-GS Gliadin Genetic diversity |
| 基金项目:国家自然科学基金项目（31571650）；陕西省重点科技创新团队计划支持项目（2014KCT-25）；西北农林科技大学唐仲英育种基金项目 |
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| 中文摘要: |
| 为挖掘优良的小麦种质资源，以引进的21份印度小麦种质为材料，利用SDS-PAGE和A-PAGE技术，分析了其高分子量谷蛋白亚基和醇溶蛋白组成。结果表明，在21份印度小麦材料中出现了12种HWM-GS亚基类型和14种亚基组合，其中有 3种亚基类型（Null、1、2*）在 Glu-A1 位点，4种亚基类型（ 7+8，17+18，7，7+9，13+16）在 Glu-B1 位点，4种亚基类型（ 5+10，2+12，4+12 ，5+12）在 Glu-D1 位点。1、2+12优质亚基的比例相对较高,均为47.6%。小麦HWM-GS亚基组合1/7+9/2+12在所有亚基组合类型中出现频率高达19.0%。大多数材料的品质得分为7、8分，平均7.52分。 共分离出32种迁移率不同的醇溶蛋白谱带，2和3号带出现频率最高,分别为95.24% 和90.48%。DA 7200近红外分析仪初步分析结果表明，这21份印度小麦种质资源品质指标相对偏低。 |
| 英文摘要: |
| In order to discover excellent germplasm resource, the HMW-GS composition and gliadin composition of 21 Indian wheat germplasms were analyzed by SDS-PAGE and A-PAGE. The results showed that there were 12 types and 14 types of subunit combinations in total. Among them, there were 3 types of subunits (Null, 1, 2*) at Glu-A1 locus, 4 types of subunits (7+8, 17+18, 7, 7+9, 13+16) at Glu-B1 locus, and 4 types of subunits (5+10, 2+12, 4+12, 5+12) at Glu-D1 locus, respectively. The frequency of high quality subunits (1, 2+12)reached up to 47.6%. Subunit combination 1/7+9/2+12 showed the highest frequency (19.0%). The quality score ranged from 7 to 8, with the average of 7.52, among all subunit combinations. There were 32 protein bands with different mobility in all gliadin bands, among which band 2 and 3 had higher frequency of 95.24% and 90.48%. Preliminary quality properties analysis of twenty-one Indian wheat germplasm were conducted by DA 7200 near infrared spectroscopy, and it showed that most quality indices of these materials were low. |
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