| 王永霞,许为钢,胡 琳,李 艳, 齐学礼,王会伟,李小博.外源玉米pepc基因对小麦C3、C4途径相关基因表达的效应[J].麦类作物学报,2016,36(1):1 |
| 外源玉米pepc基因对小麦C3、C4途径相关基因表达的效应 |
| Effect of pepc Gene from Maize on Photosynthesis-related Genes Expression in Transgenic Wheat |
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| DOI：10.7606/j.issn.1009-1041.2016.01.01 |
| 中文关键词: 磷酸烯醇式丙酮酸羧化酶 转基因小麦 基因表达 净光合速率 |
| 英文关键词:Phosphoenolpyruvate carboxylase(PEPC) Transgenic wheat Gene expression Net photosynthetic rate |
| 基金项目:国家自然科学基金项目(31371707); 国家转基因生物新品种培育科技重大专项(2011ZX08002-003/005); 国家现代产业技术体系专项(CARS-3-1-19); 国家科技支撑计划项目(2011BAD07B01) |
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| 中文摘要: |
| 为进一步探讨外源玉米pepc基因改良小麦光合性能的机制，以基因枪介导T4代的转玉米pepc基因小麦为试验材料，研究了抽穗期和灌浆期外源pepc基因对小麦内源光合相关酶基因表达的影响，并分析了转基因小麦的光合生理特性及其产量性状表现。结果表明，小麦抽穗期，pepc基因的表达上调了小麦C4微循环ppdk基因(丙酮酸磷酸双激酶基因)、nadp-me基因(NADP-苹果酸酶基因)、ca基因(碳酸酐酶基因)和C3循环rbcL基因(Rubisco大亚基基因)的表达；小麦灌浆期，pepc基因的表达仍显著上调C4微循环ppdk基因和nadp-me基因的表达，而ca基因和C3循环rbcL基因、rbcS基因(Rubisco小亚基基因)的表达量与对照差异不大。相应的酶活性在转基因植株中比对照有所提高，灌浆期增幅最大。两个测定时期的转基因小麦旗叶净光合速率(Pn)均比对照显著提高，在灌浆期增幅最大，比对照提高10.35%~22.77%。产量性状方面，转基因小麦的单穗粒数和收获指数均有所提高。上述研究结果表明，玉米C4型pepc基因导入小麦后，促进了小麦原有的C4循环，从而提高了小麦的光合效率和籽粒产量。 |
| 英文摘要: |
| To investigate how pepc gene from maize regulates the expression of endogenous photosynthesis-related genes in transgenic wheat plants,T4 transgenic wheat lines were developed by particle bombardment.The expression level of photosynthesis-related genes in the flag leaf was investigated by using PCR test,qRT-PCR and enzyme activity analysis.The results showed that exogenous gene pepc up-regulated the expression of several wheat endogenous genes,i.e.,ppdk（Pyruvate orthophosphate dikinase）,nadp-me（NADP-malic enzyme）,ca（Carbonic anhydrase）and rbcL（Rubisco large subunit）at heading stage.At filling stage,the expression levels of pepc,ppdk and nadp-me in transgenic plants were increased,whereas there was no change in the expression levels of ca,rbcL and rbcS（Rubisco small subunit） gene in transgenic plants.In transgenic plants,the enzyme activities of the corresponding genes were higher than those in untransformed plants and the increase rate reached the maximum at filling stage.Net photosynthetic rate(Pn) of transgenic plants were 10.35%-22.77% higher than that of untransformed plants at filling stage,with a maximum increase rate.Individual plant yield components analysis indicated that the grain number per spike and harvest index of transgenic plants were higher than those of untransformed plants.Introduction of pepc gene from maize into wheat plants resulted in up-regulation of both C3 and C4 photosynthetic enzymes,and increased corresponding enzyme activity,and improved photosynthetic capacity and grain yield.The results obtained from this study gave us a better understanding on the regulation mechanism of maize pepc gene in wheat for improving their photosynthesis ability. |
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