| 白升升,马 丽,王晓龙,马棫灵,张晓科,陈东升.SDS-PAGE与分子标记相结合分析宁夏小麦HMW-GS组成与变化特点[J].麦类作物学报,2015,35(12):1658 |
| SDS-PAGE与分子标记相结合分析宁夏小麦HMW-GS组成与变化特点 |
| Analysis of HMW-GS Composition Using SDS-PAGE and Molecular Markers in Ningxia Wheats |
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| DOI：10.7606/j.issn.1009-1041.2015.12.08 |
| 中文关键词: 小麦 宁夏 HMW-GS SDS-PAGE 分子标记 |
| 英文关键词:Wheat Ningxia HWM-GS SDS-PAGE Molecular makers |
| 基金项目:宁夏农业育种专项；宁夏科技支撑计划（国际科技合作）项目（2013ZYH196）；宁夏农林科学院自主研发项目（NKYG-13-02）；宁夏自然科学基金项目（NZ13108）；西北农林科技大学唐仲英育种基金项目 |
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| 中文摘要: |
| 为了建立准确有效小麦HMW-GS的检测方法，提高优质小麦品种鉴定和筛选效率，以已知HMW-GS组成的16份小麦品种为对照，优化完善SDS-PAGE结合分子标记检测小麦HMW-GS的方法，并对103份宁夏小麦品种进行了验证和分析。结果表明，8.5%的分离胶可以有效区分除了 Glu-B1位点的7*、7OE与8*亚基之外的其他亚基，分辨效果优良；SDS-PAGE结合 Bx7OE、 Bx7*/Bx7和 By8基因的分子标记可以准确鉴定小麦HMW-GS。在103份宁夏小麦品种中，发现15种HMW-GS和29种组合类型；首次在该地区小麦品种的 Glu-B1位点检测出了携带7OE、7*和8*亚基的品种；1/17+18/5+10为当地小麦HMW-GS的优势亚基组合类型，占20%。自1970年至2010年，HMW-GS的优质亚基（1、17+18和5+10）的出现频率呈明显增长趋势；宁夏小麦的HMW-GS种类和优质亚基出现频率随品种更换呈增加趋势。综上所述，分离胶浓度为8.5% 的SDS-PAGE和 Bx7OE、 Bx7*/Bx7和 By8分子标记的方法可准确有效地检测小麦HMW-GS组成，此方法可用于优质小麦品种的鉴定和筛选。 |
| 英文摘要: |
| In order to build an accurate and effective method to detect wheat high molecular weight glutenin subunits (HMW-GS) and to improve the efficiency of identification and screening of advanced wheat varieties, the known HMW-GS composition was analysed using SDS-PAGE and molecular markers in 16 control cultivars. And then 103 Ningxia wheat varieties were tested by the optimal method. The results showed that of 8.5% separating gel in SDS-PAGE could distinguish 16 control varieties with known HMW-GS composition except 7OE, 7* and 8 subunit at Glu-B1 locus. Molecular markers that could identify genes Bx7OE, Bx7*/Bx7 and By8.There were 15 HMW-GS and 29 subunit compositions in 103 Ningxia wheat varieties.7OE, 7* and 8* subunits were firstly detected in Ningxia wheat varieties. And the dominant composition was 1/17+18/5+10 with the frequency distribution of 20%.The frequency of subunits (1, 17+18 and 5+10) associated with good quality had significantly increasing tendency from 1970 to 2010. There was an increasing tendency in HMW-GS types and frequency of quality HMW-GS with the replacement of wheat cultivars in Ningxia. In short, the results indicated that combining 8.5% separating gel in SDS-PAGE and molecular markers that could identify genes Bx7OE, Bx7*/Bx7 and By8 is an accurate and effective method to detect HMW-GS, and could be used for the identification and screening of wheat varieties for quality breeding. |
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