The composition of high and low molecular weight glutenins and 1B/1R translocation is key information on wheat quality breeding. Using a total of 25 DNA molecular markers, including 7 markers specific to HMW GS, 17 to LMW GS and 1 to 1B/1R translocation, 105 Sichuan wheat cultivars released since 2000 were identified. The results showed that: (1) For HMW GS, 2 cultivars contained Ax2* at Glu A1 locus, with a frequency of 1.9%. At Glu B1 locus, 73 cultivars had Bx7, 26 carried Bx20, 4 possessed Bx17, 45 had By8 and 30 carried By9, with a percentage of 69.5%, 24.8%, 3.8%, 42.9% and 28.6%, respectively. At Glu D1 locus, 65 cultivars contained Dx5, with a frequency of 61.9%. (2) For LMW GS, the genes frequencies of the Glu A3a, Glu A3b, Glu A3c, Glu A3d, and Glu A3f at Glu A3 locus were 1.9%,1.9%,60.0%, 27.6% and 8.6%, respectively.At Glu B3 locus, the frequencies of the Glu B3b, Glu B3d, Glu B3f, Glu B3g and Glu B3i were 17.1%, 9.5%, 1.0%, 71.4% and 1.0%, respectively. (3) Thirty seven cultivars with 1B/1R translocation were detected, with a frequency of 34.3%. (4) 2 (3.8%) and 15 (14.3%) cultivars were found with a combination of 6 or 5 genes of Ax2*, Bx7, By8, Dx5, Glu A3d, Glu B3d, Glu A3b, Glu B3b and non 1B/1R translocation. These cultivars can be used as parents in wheat breeding for introducing genes with better quality potential related to gluten quality of Sichuan wheat cultivars. |