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李式昭,郑建敏,伍 玲,李 俊,蒲宗君,朱华忠.四川小麦品种高、低分子量麦谷蛋白基因和1B/1R易位的分子标记鉴定[J].麦类作物学报,2014,34(12):1619
四川小麦品种高、低分子量麦谷蛋白基因和1B/1R易位的分子标记鉴定
Identification of High and Low Molecular Weight Glutenin Genes and 1B/1R Translocation in Sichuan Wheat Cultivars with Molecular Markers
  
DOI:10.7606/j.issn.1009-1041.2014.12.04
中文关键词:  小麦  高分子量麦谷蛋白  低分子量麦谷蛋白  1B/1R易位  分子标记
英文关键词:Wheat  High molecular weight glutenin  Low molecular weight glutenin  1B/1R translocation  Molecular marker
基金项目:四川省财政基因工程论文基金项目;四川省农作物育种攻关项目;国家“863”计划项目(2012AA101105);国家自然科学基金项目(NSFC31271724);四川省科技支撑项目(2011NZ0098 16);四川省青年科技创新研究团队项目(2014TD0014
作者单位
李式昭,郑建敏,伍 玲,李 俊,蒲宗君,朱华忠 (四川省农业科学院作物研究所/农业部西南地区小麦生物学与遗传育种重点实验室四川成都 610066) 
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中文摘要:
      为给四川小麦品质育种提供参考信息,利用7个HMW GS、17个LMW GS和1个1B/1R易位的特异分子标记,对105份2000年后育成的四川小麦品种进行上述基因检测。结果表明:(1)针对HMW GS,在 Glu A1位点,含 Ax2*的品种有2份,频率为1.9%;在 Glu B1位点,含 Bx7Bx20Bx17By8By9的品种分别有73、26、4、45和30份,频率分别为69.5%、24.8%、3.8%、42.9%和28.6%,未检测到含 Bx7OE的品种;在 Glu D1位点,含 Dx5的品种有65份,频率为61.9%。(2)针对LMW GS,在 Glu A3位点,含 Glu A3aGlu A3bGlu A3cGlu A3dGlu A3f的品种分别有2、2、63、29和9份,频率分别为1.9%、1.9%、60.0%、27.6%和8.6%,未检测到含 Glu A3eGlu A3g的品种;在Glu B3位点,含Glu B3bGlu B3dGlu B3fGlu B3gGlu B3i的品种分别有18、10、1、75和1份,频率分别为17.1%、9.5%、1.0%、71.4%和1.0%,未检测到含Glu B3aGlu B3cGlu B3eGlu B3h的品种。(3)含1B/1R易位的品种有36份,频率为34.3%。(4)组合6种和5种以上优质基因的品种分别有2份(频率为3.8%)和15份(频率为14.3%)。可利用这些品种作为亲本,在四川小麦品质育种中逐步导入优质基因 Ax2*Bx7By8Dx5Glu A3dGlu B3dGlu A3bGlu B3b,并淘汰1B/1R易位,优化四川小麦面筋优质基因组成。
英文摘要:
      The composition of high and low molecular weight glutenins and 1B/1R translocation is key information on wheat quality breeding. Using a total of 25 DNA molecular markers, including 7 markers specific to HMW GS, 17 to LMW GS and 1 to 1B/1R translocation, 105 Sichuan wheat cultivars released since 2000 were identified. The results showed that: (1) For HMW GS, 2 cultivars contained Ax2* at Glu A1 locus, with a frequency of 1.9%. At Glu B1 locus, 73 cultivars had Bx7, 26 carried Bx20, 4 possessed Bx17, 45 had By8 and 30 carried By9, with a percentage of 69.5%, 24.8%, 3.8%, 42.9% and 28.6%, respectively. At Glu D1 locus, 65 cultivars contained Dx5, with a frequency of 61.9%. (2) For LMW GS, the genes frequencies of the Glu A3a, Glu A3b, Glu A3c, Glu A3d, and Glu A3f at Glu A3 locus were 1.9%,1.9%,60.0%, 27.6% and 8.6%, respectively.At Glu B3 locus, the frequencies of the Glu B3b, Glu B3d, Glu B3f, Glu B3g and Glu B3i were 17.1%, 9.5%, 1.0%, 71.4% and 1.0%, respectively. (3) Thirty seven cultivars with 1B/1R translocation were detected, with a frequency of 34.3%. (4) 2 (3.8%) and 15 (14.3%) cultivars were found with a combination of 6 or 5 genes of Ax2*, Bx7, By8, Dx5, Glu A3d, Glu B3d, Glu A3b, Glu B3b and non 1B/1R translocation. These cultivars can be used as parents in wheat breeding for introducing genes with better quality potential related to gluten quality of Sichuan wheat cultivars.
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