| 李婷婷，张芸芸，孙瑜琳, 王艳婷，王美玲，胡世玮，汪 勇，史 雪，权 力，王中华.二穗短柄草表皮蜡质组成分析及蜡质显微结构观察[J].麦类作物学报,2014,34(7):969 |
| 二穗短柄草表皮蜡质组成分析及蜡质显微结构观察 |
| Cuticular Wax Composition and Microscopic Structure of Different Organs of Brachypodium distachyon |
| |
| DOI：10.7606/j.issn.1009-1041.2014.07.15 |
| 中文关键词: 二穗短柄草 表皮蜡质 成分 晶体状态 |
| 英文关键词:Brachypodium distachyum Cuticular wax Wax crystal Scanning electron microscope |
| 基金项目:国家自然科学基金项目（31271794）;全国博士学位论文作者专项资金资助项目（201176）;西北农林科技大学科研启动项目（Z111021102） |
|
| 摘要点击次数: 1199 |
| 全文下载次数: 918 |
| 中文摘要: |
| 为了解二穗短柄草各器官表皮蜡质组成及其晶体结构，利用气相色谱（GC）技术对二穗短柄草穗下茎、叶、穗和旗叶鞘的表皮蜡质成分进行了分析，并对其表皮蜡质晶体结构进行扫描电镜观察。结果表明，从二穗短柄草表皮蜡质中鉴定出18种化合物成分，主要为初级醇、烷烃、醛和脂肪酸。其中，初级脂肪醇、脂肪醛和烷烃是其蜡质的主要成分，约占总蜡质含量的96%。不同器官的蜡质成分比较相似，均以初级脂肪醇的含量最高，其次是脂肪醛、烷烃和脂肪酸。不同器官间蜡质总含量有显著差异，其中穗和旗叶的蜡质含量比较高，分别为13.6和9.8 μg·cm-2，而倒三叶和旗叶叶鞘最低，分别为4.9和4.7 μg·cm-2。蜡质成分碳链长度的分布范围主要为C22~C32，其中以C26分布最多。初级醇中C26醇的含量最高，分别占叶片、穗和叶鞘中醇含量的94.6%、95.7%、91.4%，占穗下茎中醇含量的76.5%。烷烃中以C29烷烃含量最高，大约占60%左右；其次是C31烷烃，含量在叶片蜡质中大约为30%，穗下茎中是34.2%。醛中C26醛含量最高，约占叶片、穗和叶鞘中醛含量的91.5%~94.6% ,占穗下茎中醛含量的74.4%。脂肪酸中C20和C22脂肪酸的含量较高，C26和C28脂肪酸的含量比较低。经扫描电镜观察，各器官表面的蜡质晶体结构均为片状。 |
| 英文摘要: |
| The cuticular wax compositions were analyzed by GC FID/MS on in different organs of Brachypodium distachyum(Bd21), including peduncle, leaf, spike and leaf sheath, and the wax crystal structure was observed by scanning electron microscope. The results showed that 18 compounds were identified by GC MS in the wax and could be classified as primary alcohols, alkanes, aldehydes and fatty acids. The main components of the wax were primary alcohols, alkanes and aldehydes, which accounted for 96% of total wax content. The wax compositions in different organs were similarly with the highest content of alcohols, then followed by alkanes, aldehydes and fatty acids. The total wax contents were significantly different among different organs, the wax content of spike (13.6 μg·cm-2) and flag leaf (9.8 μg·cm-2) was higher than that of top third leaf (4.9 μg·cm-2) and flag leaf sheath (4.7 μg·cm-2). The carbon chain length distribution of wax compounds ranged from C22 to C32 and dominated by C26. In primary alcohols, the content of C26 alcohol was the highest, which accounted for 94.6%, 95.7%, 91.4% and 76.5% of total primary alcohols in leaf, spike, leaf sheath and peduncle, respectively. In alkanes, the content of C29 alkane (approximate 60%) was the highest, then C31 alkane, which accounted for 30% in leaf and 34.2% in peduncle. For aldehydes, the content of C26 aldehyde was the highest, which accounted for 91.5%~94.6% of total aldehydes in leaf, spike and leaf sheath, and 74.4% in peduncle. For fatty acids, the contents of C20 and C22 were higher and C26 and C28 acids were lower. Based on the observation of scanning electron microscope the crystal structures of wax in the surfaces of different organ were similar in type of platelets. |
|
查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
基本信息
