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作者	单位
尚毅，华为，朱靖环，贾巧君，汪军妹，杨建明	(浙江省农业科学院作物与核技术利用研究所，国家大麦改良中心，浙江杭州 310021)

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中文摘要:

为给保存和开发利用大麦地方品种资源提供参考信息，采用1个Nud基因特异标记和28 对SSR标记，以23份浙江省皮大麦地方品种及江苏省的1份裸大麦和1份皮大麦地方品种作为参照，研究了66份浙江省裸大麦地方品种的遗传多样性。结果表明，所有检测的裸大麦地方品种在Nud基因位点都存在17 kb的片段缺失，表明浙江省裸大麦地方品种裸粒性状是单一起源的。28对SSR引物共检测到91个等位基因，其中17个等位基因为裸大麦特有，3个等位基因为皮大麦特有。裸大麦的平均等位基因数、有效等位基因数、Shannon指数、Nei’s基因多样性指数和多态性信息含量均高于皮大麦，表明浙江省裸大麦地方品种的遗传多样性比皮大麦地方品种更为丰富。聚类分析将裸大麦和皮大麦分别聚为1大类，表明浙江省裸大麦地方品种和皮大麦地方品种的遗传差异较大，而裸大麦又可分为3小类，但它们与品种地理来源无任何联系。

英文摘要:

One marker specific to Nud and 28 SSR markers were used to analyze the genetic diversity of 66 hulless barley landraces accessions from Zhejiang province, with 23 hulled barley accessions from Zhejiang province, 1 hulless barley accession and 1 hulled barley accession from Jiangsu province being as the references. The results revealed that all hulless landraces were characterized by a 17 kb deletion at Nud locus, indicating the monophyletic origin of these hulless barley landraces. Totally 91 alleles were identified among these landraces using 28 SSR markers, with 17 alleles being specific for hulless barley landraces and 3 alleles being specific for hulled barley landraces. Hulless barley landraces had higher average alleles, average effective alleles, Shannon information index (I), Nei’s gene diversity index (H) and polymorphism information content (PIC) than hulled barley landraces, indicating that hulless barley landraces had a wider genetic diversity. Cluster analysis showed that hulless and hulled barley landraces could be clustered into independent group, indicating higher genetic difference between these types of barley landraces. The hulless barley group could be further divided into 3 sub groups, although there was no close association between accession groups and their geographical origins.

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