Semi-winter wheat cultivar Yumai 49 and weak spring wheat cultivar Taikong 6 were used in a field experiment with 3 nitrogen application levels of 90 (N1), 180 (N2), 270 (N3) kg·hm-2 to investigate the effects of nitrogen application on the spikelet length of main stem, development of the florets at different positions of the 9th spikelet, spikelet differentiation per spike, number of male sterile spikelets and spikelet setting rate, yield and its components. Results showed that along with wheat development, the spikelet length of the main stem response to nitrogen application obviously, as with the increasing of nitrogen application amount, Yumai 59 showed N3>N2>N1, Taikong 6 showed N2>N3>N1. The 3rd~6th floret development of 9th spikelet of Yumai 49 significantly responsed to the nitrogen application amount, the differentiation degree increased with the increasing of nitrogen application amount.The 3rd~6th floret development of the 9th spikelet of Taikong 6 also obviously responsed to the nitrogen application amount, as the 3rd floret development at early period in N2 was earlier than in N1 and N3 treaments, and at middle and later period in N2 and N3 treatemnts was earlier than in N1; the 4th~6th floret differentiation degree showed the trend of N2>N3>N1. Obvious differences among different nitrogen application amount were observed in the number of spikelet differentiation per spike, male sterile spikelet, spikelet setting rate, yield and spike number, and also between the two wheat cultivars.The number of spikelet differentiation per spike, seed setting rate, yield and spike number of Yumai 49 showed as N3>N2>N1, while those of Taikong 6 showed as N2>N3>N1; the spikelet setting rate of Yumai 49 showed as N3 |