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作者	单位
苏佳妮,武军,赵继新,王秀娟,张军,贺晓岚,刘淑会,杨群慧,陈新宏	(西北农林科技大学农学院，陕西杨凌 712100)

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中文摘要:

为给小麦-华山新麦草远缘杂交育种提供材料和理论基础，对普通小麦与华山新麦草杂交后代中选育出的材料24-6进行了形态学观察、细胞学检测和原位杂交(GISH)分析。结果表明：(1)通过根尖细胞学鉴定，该材料染色体数为42条，2n=42；(2)以华山新麦草Ns基因组DNA为探针，普通小麦7182基因组DNA为封阻，根尖与花粉母细胞原位杂交(GISH)显示该材料携带有2条可以配对的华山新麦草染色体臂，确定24-6为稳定的小麦-华山新麦草易位系；(3)田间观察发现24-6植株紧凑，叶色深绿，籽粒白色、呈半角质，比其母本（普通小麦7182）株高略有降低，穗长、小穗数、穗粒数、千粒重、分蘖数都有所增加。

英文摘要:

Morphological observation, cytological detection and genomic in situ hybridization (GISH) analysis were carried out on a translocation line 24-6 from the offsprings between Wheat-Psathyrostachys huashanica in the present paper. The results showed that：(1)This material had 42 chromosomes（2n = 42）as cytology identification of root tip cells；(2) Using the genomic DNA of Psathyrostachys huashanica Ns as a probe,and the genome DNA of common wheat 7182 as blocking， GISH analysis with root tips and pollen mother cells identified two Psathyrostachys huashanica chromosomes which paired with each other in this line；(3) The field observation found that the line 24-6 had dark green leaf color, white grain (half horniness), slightly shorter plant height than the female parent. The spike length, spikelet number, grain number per spike, and tiller number were all increased. Consequentially, 24-6 is a stable wheat -Psathyrostachys huashanica translocation line, which could be exploited as an intermediate material in wheat genetic and breeding programs.

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