| 曹 东，张雪婷，王世红，王东霞，赖 勇，孟亚雄，王化俊，杨文雄.104份甘肃小麦品种脂肪氧化酶和多酚氧化酶活性基因等位变异的检测[J].麦类作物学报,2014,34(4):467 |
| 104份甘肃小麦品种脂肪氧化酶和多酚氧化酶活性基因等位变异的检测 |
| Molecular Detection of Allelic Variations of Genes for Lipoxygenase and Polyphenol Oxidase in 104 Gansu Wheat Cultivars |
| |
| DOI：10.7606/j.issn.1009-1041.2014.04.06 |
| 中文关键词: 甘肃小麦 脂肪氧化酶 多酚氧化酶 等位变异 功能标记 |
| 英文关键词:Gansu wheat Lipoxygenase Polyphenol oxidase Allelic variations Functional marker |
| 基金项目:国家自然科学基金项目（30771331，31171558）；甘肃省科技重大专项（1203NKDF018）；甘肃省自然科学基金项目（1208RJ2A136）。 |
|
| 摘要点击次数: 1711 |
| 全文下载次数: 1208 |
| 中文摘要: |
| 面粉和面制品的色泽是评价小麦品质的重要指标。小麦脂肪氧化酶（LOX）和多酚氧化酶（PPO）活性对面粉白度及面制品的色泽具有重要影响。为给甘肃省小麦品质育种提供参考依据，以104份甘肃省育成的小麦品种为材料，利用功能标记 LOX16、 LOX18、 PPO18、 PPO16和 PPO29检测 TaLox B1、 Ppo A1及 Ppo D1位点的等位变异，分析甘肃小麦品种资源中LOX和PPO活性基因的组成和分布特点。结果表明，在甘肃小麦中，等位变异 TaLox B1a和 TaLox B1b的频率分别为22.12%和77.88%；其中，甘肃冬小麦品种高LOX活性等位变异 TaLox B1a的分布频率（30.56%）高于春小麦（3.13%）。等位变异 Ppo A1a、 Ppo A1b、 Ppo D1a和 Ppo D1b的频率分别为49.04%、50.96%、50.96%和49.04%；两个PPO基因的等位变异组合 Ppo A1a/ Ppo D1b、 Ppo A1a/ Ppo D1a、 Ppo A1b/ Ppo D1b和 Ppo A1b/ Ppo D1a的分布频率依次为28.85%、20.19%、20.19%和30.77%。说明在甘肃小麦品种中，低LOX活性等位变异（ TaLox B1b）品种比例较高；低PPO活性等位变异（ Ppo A1b、 Ppo D1a）品种分布比例略高于高PPO活性类型；其中，32份小麦品种在 TaLox B1、 Ppo A1和 Ppo D1三个位点同时含有高LOX活性和低PPO活性的等位变异。 |
| 英文摘要: |
| Flour color and appearance color of flour products are the important indicators for evaluating wheat quality. The activities of wheat lipoxygenase (LOX) and polyphenol oxidase (PPO) have relatively important effects on the flour whiteness and color of flour products. In this study, 104 wheat varieties released in Gansu province were used as experimental materials to detect the allelic variation of lipoxygenase gene TaLox B1 and polyphenol oxidase gene Ppo A1 and Ppo D1 by using function marker LOX16， LOX18， PPO18， PPO16 and PPO29, in order to further investigate the distribution characteristics of the three genes in wheat varieties from Gansu. The results showed that, the frequencies of cultivars with genes of TaLox B1a and TaLox B1b were 22.12% and 77.88%, respectively; the distribution frequency of LOX high activity gene TaLox B1a in winter wheat varieties from Gansu (30.56%) was higher than that in spring wheat (3.13%). The frequencies of cultivars with genes of Ppo A1a， Ppo A1b， Ppo D1a and Ppo D1b were 49.04%, 50.96%, 50.96% and 49.04%, respectively. Frequencies of allelic combinations of PPO genes for Ppo A1a/ Ppo D1b, Ppo A1a/ Ppo D1a, Ppo A1b/ Ppo D1b and Ppo A1b/ Ppo D1a were 28.85%, 20.19%, 20.19% and 30.77%, respectively. Wheat cultivars recently released in Gansu province had a relatively high proportion of LOX low activity gene TaLox B1b. and the genes of low PPO activity were dominant. Among those varieties, 32 varieties contained the desired allelic variation for LOX and PPO genes. |
|
查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
基本信息
