| Winter wheat cultivar Dongnongdongmai 1 was used to identify the relationship between ABA and the expression and regulation of miRNA related with the development and cold tolerance. Leaves were sprayed with 10-5 mol·L-1 ABA when seedlings were at the 3 true leaf stage (1 L·4 m-2),tillering nodes were sampled when temperature dropped to about 4℃,0℃,-10℃ and-25℃ in the field,and the expressions of 6 higher abundance and significantly differentially expressed miRNA (miR165,miR166,miR319,miR5070,miR5139 and miR3704) selected from our previous small RNA libraries were studied by real time quantitative PCR. The results indicated that the expressions of miR165,miR166,miR319 and miR5139 increased at first and then decreased in the control group as the temperature decreased,and the expression of miR5070 showed an increase decrease increase trend,and the expression of miR3704 increased; while the expression patterns of miR165,miR166,miR5070,miR5139 and miR3704 all appeared a decrease increase decrease trend,and the expression of miR319 decreased at first and increased in the ABA treated group,and it indicated that ABA could change the expression patterns of each miRNA. The expressions of the miRNAs in ABA treated samples compared to the control.were up regulated at 4 ℃ and -10 ℃,and down regulated at 0℃,and all miRNAs were up regulated except that miR5070 was down regulated at -25 ℃,Those various changes at such low temperatures may be associated with the function of the miRNA target genes. |
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