| 徐先良，赖 勇，王鹏喜，范贵强，汪军成，王 晋，孟亚雄，李葆春，马小乐，王化俊(1.甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室，甘肃兰州 730070；2.甘肃农业大学农学院，甘肃兰州 730070； 3.甘肃农业大学生命科学技术学院，甘肃兰州 730070).大麦亲本材料农艺性状鉴定及遗传多样性分析[J].麦类作物学报,2013,33(4):640 |
| 大麦亲本材料农艺性状鉴定及遗传多样性分析 |
| Identification of Agronomic Traits and Genetic Diversity Analysis of Barley Parent Materials |
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| DOI：10.7606/j.issn.1009-1041.2013.04.004 |
| 中文关键词: 大麦 SSR 农艺性状 遗传多样性 |
| 英文关键词:Barley SSR marker Agronomic traits Genetic diversity |
| 基金项目:国家自然科学基金面上项目（30771331，31171558）；国家大麦青稞产业体系项目（CARS-05）。 |
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| 中文摘要: |
| 为了解大麦亲本材料间的遗传差异及农艺性状特征，利用46对SSR引物对61份大麦材料进行遗传多样性分析，并鉴定了其田间农艺性状。结果表明，株高最高和最矮的材料分别为苏盐0014和Z122V032W；穗长最长和最短者分别为阿恩特13和富士二条；BYT CRA3的穗粒数最多。不同农艺性状的相关性分析表明，株高和第二节间长，第一节间长和第二节间长，穗长和穗粒数均具有极显著的相关性。SSR分析结果表明，46对SSR引物在61份材料中共检测到117个等位变异，单个引物可检测到1~5个等位变异，平均每个引物可检测到2.5个。聚类分析结果表明，参试材料遗传相似系数(GS)为0.572 6~0.954 4，平均值为0.753 0。在GS为0.726 0处，61份材料可聚为5大类。大多数国内材料被聚为一类，国外材料与国内材料遗传差异较大。 |
| 英文摘要: |
| In order to know the genetic differences and the agronomic characteristics of barley parent materials and provide useful information for parent combinations, the genetic diversity of 61 barley parent materials were analyzed by 46 SSR markers and agronomic traits were identified in the field. The results showed that Suyan 0014 was the highest and Z122V032W was the lowest in plant height. The spike length of Arndt 13 was the longest, and Fushi Ertiao was the shortest. The grain number per spike of BYT CRA3 was the most in 61 materials. Correlation analysis of different agronomic traits indicated that there were high correlation between plant height and second internode, the same as first internode and second internode,the same as first internode and second internode,and also spike length was significantly related with grain number per spike. SSR analysis showed that a total of 117 alleles were detected in 61 materials by the 46 markers. Each marker could detect 1~5 alleles with 2.5 alleles in average. Clustering analysis revealed that the genetic similarity coefficient (GS) ranged from 0.572 6 to 0.954 4, with the mean of 0.753 0. The 61 materials could be clustered into 5 groups at the level of 0.726 0. |
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