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作者	单位
马勇，邵立刚，王岩，李长辉，车京玉，高凤梅，张起昌，刘宁涛，邹东月，王志坤	（黑龙江省农业科学院克山分院，黑龙江克山 161606）

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中文摘要:

为了给小麦抗秆锈病品种的选育提供理论依据，利用与Sr33紧密连锁的SSR引物BARC152对国内外引进的58份抗Ug99的春小麦品种以及黑龙江省主栽小麦品种18份进行SSR检测。结果表明，国内外引进的58份抗Ug99春小麦材料中仅有1份材料含有Sr33，占检测材料的1.7%；黑龙江省18份主栽春小麦品种中有3份材料中含有Sr33，占检测材料的16.7%。表明Sr33在引进的抗Ug99材料和黑龙江省主栽小麦品种中占有的比例均很少，今后要加强Sr33在小麦抗病育种中的应用。

英文摘要:

Sr33 is an important stem rust resistant gene, not only resistant to the domestic main stem rust races, but also for the new virulent pathogen Ug99 (TTKSK) and its variant races TTKST and TTTKS. The aim of this study was to provide theoretical basis for the stem rust resistant gene Sr33 in wheat breeding programs. The microsatellite marker BARC152, which closely linked to Sr33 with good repeatability and high accuracy, was used to detect the presence of Sr33 gene in spring wheat germplasm and cultivars resistant to Ug99. The results indicated that only 1 genotype carried the resistant gene among the 58 improved Ug99 resistant spring wheat cultivars introduced from abroad, with a frequency of 1.7%, and 3 genotypes carried this resistant gene among the 18 main wheat cultivars in Heilongjiang province, with a frequency of 16.7%. It reflected that Sr33 was in a smaller proportion in introduced cultivars and main wheat cultivars in Heilongjiang, and much attention should be paid to the use of Sr33 in wheat breeding program.

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