| Drought stress has adverse effects on wheat production and quality. In order to investigate the consequences of drought stress during grain filling on the accumulation of seed proteins, the cultivar Gaocheng 8901 was subjected to drought stress during grain filling. A total of 130 protein spots were detected differentially expressed. Among those, 57 spots were successfully identified by MALDI TOF TOF MS, 29 of which were found to be up regulated, whereas 19 were down regulated, 7 were specific expressed and 2 were silenced. These proteins included ADP glucose pyrophosphorylase, alpha amylase inhibitor, peroxiredoxin, beta amylase, protein disulfide isomerase, serpin, superoxide dismutase, globulin, cold regulated protein, fructose bisphosphate aldolase, glucose and ribitol dehydrogenase and eukaryotic translation initiation factor 5A1. These proteins were involved in metabolism, energy, protein processing, disease and defense, transcription and unknown functions. Some were enzymes involved in different metabolic pathways of plants as well as some were stress related proteins. Some proteins identified were existed in multiple spots with different pIs and molecular masses, implying that post translational modifications (PTMs) of proteins may involve in wheat drought stress resistance. The results provided new insights into biochemical mechanisms for wheat drought tolerance during grain filling. |
基本信息
