| 牛国阳,陈庆富.几种小麦雄性不育系及其保持系、恢复系的酯酶同工酶比较[J].麦类作物学报,2012,32(4):660 |
| 几种小麦雄性不育系及其保持系、恢复系的酯酶同工酶比较 |
| A Comparative Study of Esterase Isozyme in Flag Leaves and Young Spikelets among Some Common Wheat CMS Lines and Their Maintainers and Restorers |
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| DOI：10.7606/j.issn.1009-1041.2012.04.011 |
| 中文关键词: 普通小麦 胞质雄性不育系 旗叶 幼小穗 酯酶同工酶 |
| 英文关键词:Common wheat Cytoplasm male sterile lines Flag leaf Young spikelet Esterase isozyme |
| 基金项目:教育部新世纪人才支持计划项目（NCET 04 0913）；贵州省小麦“十一五”重大科技攻关项目（030507）。 |
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| 中文摘要: |
| 为了阐明同核不同细胞质的小麦雄性不育系、保持系、恢复系之间酯酶同工酶的异同，通过聚丙烯酰胺凝胶电泳(PAGE)对T型、Q型、AL型三种小麦雄性不育系及其保持系、恢复系等合计35个材料的孕穗期旗叶和幼小穗中的酯酶同工酶进行了研究。结果表明，保持系与恢复系在旗叶和幼小穗的酯酶同工酶上未发现明显差异。但不同不育系（TA92 8、QA92 8、AL92 8）之间以及与其同核保持系(绵阳92 8)之间存在差异。其中，Q型不育系在旗叶和幼小穗酯酶同工酶上与同核保持系一致，但T型、AL型不育系比其保持系多2条旗叶期酯酶同工酶谱带(ESTa 8,9)。上述结果说明，不育系与保持系之间的谱带差异可能与特定核质互作有关。本研究为T型、Q型、AL型不育系的区分提供了一个有效的方法。 |
| 英文摘要: |
| In order to study the differences of esterase spectrum among different cytoplasmic male sterile lines with the same nuclear and among A lines, B lines and R lines, the easterase isozymes (EST) in flag leaf and young spikelet of T , Q , AL type male sterile lines with same nuclear and their B lines, and R lines etc totally 35 common wheat lines were studied by means of PAGE analysis. The results indicated that there are no obviously differences of the esterase isozyme in the flag leaf and young spikelets between B lines and R lines. There are, however, some differences among T, Q, AL type sterile lines and between sterile lines (QA92 8, TA92 8, and AL92 8) and their B line (Mianyang 92 8). Among them, Q type sterile line has similar EST isozyme spectrum in flag leaves and young spikelets to its maintainer, and T type and AL type sterile lines have two additive bands (ESTa 8,9) in flag leaves more than their maintainer, indicating special nuclear cytoplasm interaction of the different sterile lines and an identification method for different cytoplasm sterile lines. |
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