| 马丽娟，王 翔，卫 丽，冯雅岚，任江萍，尹 钧.不同发育特性小麦品种春化基因 VRN2的序列分析[J].麦类作物学报,2012,32(4):603 |
| 不同发育特性小麦品种春化基因 VRN2的序列分析 |
| Sequences Analysis of the Vernalization Gene VRN2 in Different Development Characteristic Common Wheat (Triticum aestivum L.) |
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| DOI：10.7606/j.issn.1009-1041.2012.04.002 |
| 中文关键词: 普通小麦 春化基因 VRN2 突变 |
| 英文关键词:Common wheat Vernalization gene VRN2 Mutation |
| 基金项目:国家支撑计划重大项目（2011BAD16B07）； 河南省教育厅自然基金项目（2010B210015）。 |
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| 中文摘要: |
| 为了明确小麦春化基因 VRN2与春化发育表现型的关系，以6个不同春化发育特性的普通小麦品种为试验材料，采用PCR技术对春化基因 VRN2的CCT保守区中43个氨基酸序列进行了分析。结果表明，不同品种间 ZCCT A1的CCT功能域的序列存在差异，肥麦有R35W突变，另外5个品种均有R39C突变； ZCCT A2均存在R16C突变；B和D基因组中均未发现突变。这表明 VRN2基因编码区的等位变异主要出现在A基因组上，而B和D基因组中的 VRN2基因在目前大面积主栽品种中均为显性。 |
| 英文摘要: |
| To identify the relationship of vernalization gene VRN2 and growth habit in common wheat， The 43 amino acid sequences of the conserved CCT domain in the ZCCT1 and ZCCT2 genes of 6 wheat (Triticum aestivum) cultivars with different vernalization traits were analyzed using the sequence specific PCR amplification. The results showed that 6 cultivars had different mutation in CCT domain of ZCCT A1 gene, Feimai had R35W mutation, the other five cultivars had R39C mutation in ZCCT A1. while all of the 6 cultivars had R16C mutation in ZCCT A2 gene. B and D genomes of the 6 cultivars had no mutations. Allelic variation of VRN2 coding region is mainly refiected in the A genome, while VRN2 in B、D genome is dominant in main cultivars. |
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