| 芦 静, 胡 钰, 周安定, 梁晓东, 曹俊梅，张新忠, 黄天荣, 高永红, 吴新元.新疆小麦黄色素含量基因等位变异的分子检测及其分布[J].麦类作物学报,2012,32(2):234 |
| 新疆小麦黄色素含量基因等位变异的分子检测及其分布 |
| Molecular Detection and Distribution of Allelic Variations of the Gene for Yellow Pigment Content in Xinjiang Wheat Cultivars |
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| DOI：10.7606/j.issn.1009-1041.2012.02.008 |
| 中文关键词: 普通小麦 黄色素含量 八氢番茄红素合成酶基因 功能标记 分子标记辅助选择 |
| 英文关键词:Common wheat Yellow Pigment Content Phytoene synthase gene Psy A1 Functional marker Marker assisted selection |
| 基金项目:新疆自治区农作物生物技术重点实验室开放项目（XJYS0302 2009 03）；新疆自治区研究与技术开发计划重大专项（200731132 1）。 |
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| 中文摘要: |
| 为了明确新疆小麦材料 Psy A1和 Psy B1位点控制籽粒黄色素含量基因的组成和分布以及与黄色素含量的关系，利用7A和7B染色体上 Psy A1和 Psy B1基因的分子标记YP7A和YP7B，对326份新疆冬、春小麦材料中的等位变异进行检测。结果表明，在 Psy A1位点，含有等位基因 Psy A1a和 Psy A1b的品种(系)分别占83.74%和16.26%，两种基因型黄色素含量平均值的差异达到极显著水平(P<0.01)；在 Psy B1位点，含有等位基因 Psy B1a 和 Psy B1b的品种(系)分别占83.44%和16.56%,但两种基因型黄色素含量平均值的差异不显著(P>0.05)。不同变异组合类型的分布比例表现不同， Psy A1a/ Psy B1a所占比例最高，为69.63%；其次是 Psy A1a/ Psy B1b组合类型，为14.11%； Psy A1b/ Psy B1a组合类型为13.8%； Psy A1b/ Psy B1b所占比例最低，为2.45%，没有扩增出 Psy B1c 和 Psy B1d 基因位点。总体来看，新疆小麦高黄色素含量的等位变异类型所占比例较高，冬、春小麦黄色素含量基因型存在一定差异；YP7A可以作为一个稳定、高效的功能标记用于分子标记辅助选择，而YP7B标记则不能独立作为黄色素含量辅助选择的实用性标记。 |
| 英文摘要: |
| In order to define the distribution of allelic variations for genes Psy A1 and Psy B1 controlling wheat yellow pigment content and its relationship with yellow pigment content in Xinjiang wheat cultivars (lines), and to test the applicability of Psy A1 and Psy B1 genes, 326 wheat cultivars(lines) growing mainly in Xinjiang were tested with the functional markers YP7A and YP7B developed from the sequence polymorphisms of Psy A1 gene on chromosome 7A and Psy B1 gene on chromosome 7B. Results showed that the proportion of the allelic variations of Psy A1a and Psy A1b at Psy A1 loci in these varieties (strains) were 83.74% and 16.26%, respectively, the difference of yellow pigment content between the two genotypes was very significant (P < 0.01); the proportion of the allelic variations of Psy B1a and Psy B1b at Psy B1 loci in these varieties (strains) were 83.44% and 16.56%, respectively, the difference of yellow pigment content between the two genotypes was not significant (P < 0.05).The frequencies among allelic combinations were also different, the frequencies of allelic combinations Psy A1a / Psy B1a , Psy A1a / Psy B1b , Psy A1b / Psy B1a , Psy A1b / Psy B1b were 69.63%, 14.11%, 13.8% and 2.45%, respectively. Psy B1c and Psy B1d allele was absent in these varieties. Generally, the number of cultivars with high yellow pigment content in Xinjiang was high. There were difference in yellow pigment content between winter wheat and spring wheat. The YP7A could be directly used as stable and efficient functional markers to promoting the quality breeding efficiency, the YP7B could not be used as efficient markers for the selection of yellow pigment content independently. |
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