欢迎访问《麦类作物学报》编辑部网站

山羊草属植物是普通小麦改良过程中重要的有益基因来源，小麦的许多抗病虫、抗逆基因都来源于山羊草属植物。本研究利用杂交和回交的方法，成功获得了19株小麦卵穗山羊草衍生后代，实验选取均匀分布于小麦各条染色体的SSR标记400个，对三个亲本（中国春、卵穗山羊草和陕优225）以及19株衍生后代进行分子标记特异性分析，结果表明，341个标记可以在卵穗山羊草中扩增出条带，说明SSR标记在山羊草中位点丰富；20个标记可以在卵穗山羊草中扩增出特异条带，说明在卵穗山羊草中有不同于另外两个亲本的特异SSR位点；将20个在卵穗山羊草亲本中有特异条带的标记应用于19株衍生后代中，发现有10个标记可以在衍生后代中扩增出特异条带，说明19株衍生后代中全部含有卵穗山羊草的遗传物质，这些特异引物可以继续应用于后代的检测中。本实验还对3个亲本材料和19株衍生后代进行了成株期白粉病抗性鉴定，结果显示，中国春和陕优225都是9级高感，卵穗山羊草是0级免疫，19株衍生后代中有3株(1 1, 1 2, 1 3)分别是7、8和8级感病，其余均为0级免疫，说明衍生后代的白粉病抗性完全遗传自卵穗山羊草亲本。

英文摘要:

Aegilops species carrying the U or/and M genomes represent a prominent source of useful genes for wheat breeding. We generated 19 wheat Aegilops ovata derivatives through hybridization between common wheat and Aegilops ovata. Four hundred SSR (simple sequence repeat) markers were used to investigate the polymorphisms among parental lines, Chinese Spring, Shaanyou 225 and Aegilops ovata, of which 341 markers (85.25%) could amplify bands in Aegilops ovata, and 20 of them (5%) showed specific bands in Aegilops ovata, and were applied to investigate germplasm inheritance of Aegilops ovata in wheat Aegilops ovata derivatives. The results showed that 10 markers could amplify specific bands in the 19 derivatives, indicating that all these 19 derivatives inherited germplasms from Aegilops ovata and these 10 SSR markers could be used in further identification of next generations. We also conducted powdery mildew resistance evaluation on the 19 derivatives, and 16 of them are immune to powdery mildew like Aegilops ovata, one of their parents, but not like the other two parental lines, Chinese Spring and Shaanyou 225, which are highly susceptible to powdery mildew, indicating that the powdery mildew resistance in the 16 derivatives were explicitly inherited from Aegilops ovata.

查看全文查看/发表评论下载PDF阅读器

关闭