| 王延鹏，高 翔，陈其皎，赵万春，董 剑, 史红飞，臧 闻，李志业，李晓燕.簇毛麦新型低分子量谷蛋白亚基的分子克隆与序列分析[J].麦类作物学报,2011,31(2):29 |
| 簇毛麦新型低分子量谷蛋白亚基的分子克隆与序列分析 |
| Cloning and Sequence Analysis of Novel LMW GS Genes from Dasypyrum villosa |
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| DOI：10.7606/j.issn.1009-1041.2011.02.005 |
| 中文关键词: 簇毛麦；LMW GS 序列分析；进化关系 |
| 英文关键词:Dasypyrum villosa LMW GS Sequence analysis Evolutionary |
| 基金项目:国家自然科学基金项目(30900896)；陕西省“13115”科技创新工程重大项目(2007ZDKG 01)；农业部现代农业产业技术体系建设专项(NYCYTX 001)；中央高校基本科研业务费专项资金项目(QN2009007)。 |
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| 中文摘要: |
| 为发掘低分子量谷蛋白亚基（LMW GS）新的变异类型，通过设计引物和特异PCR扩增，从簇毛麦TA2127基因组DNA中得到14个LMW GS基因，GenBank登录号分别为HQ615147～HQ615160。序列分析表明，所有基因具有完整编码区，长度为831 bp和846 bp，可编码277和282个氨基酸残基，推导的氨基酸序列分析显示,14个基因编码的蛋白序列都缺少一段N 端保守区，且N 端序列是一种新的类型；在14个LMW GS基因中检测到19个SNPs和1个16 bp 的缺失。系统进化分析表明，簇毛麦LMW GS与ω 醇溶蛋白和HMW GS亲缘关系相对较远，与普通小麦 Glu 3的低分子谷蛋白基因进化关系相对较近。 |
| 英文摘要: |
| In order to discover new LMW GS genes and provide more important information for the further genetic improvement, fourteen novel LMW GS genes (GenBank accession number: HQ615147～HQ615160) from Dasypyrum villosa (TA2127) were amplified and cloned using PCR based strategy. The lengths of the open reading frames (ORF) of those LMW GS genes were 831 and 846 bp, encoding a mature protein of 277 and 282 amino acid residues. Comparative analysis showed that all genes lacked the conserved sequence of N terminal, and the N terminal was new types. A total of nineteen single nucleotide polymorphisms (SNPs) and a 16 bp deletion among the fourteen LMW GS genes were identified. Phylogenetic and evolutionary analysis revealed that the LMW GS genes from Dasypyrum villosa were closely related to LMW GS genes of Glu 3 and less homology was found between LMW DV genes with HMW GS and ω gliadin genes. |
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