欢迎访问《麦类作物学报》编辑部网站

作者	单位
石立旗，焦　浈，张鲁军，秦广雍	（郑州大学河南省离子束生物工程重点实验室，河南郑州４５００５２）

摘要点击次数: 1828

全文下载次数: 2349

中文摘要:

为给离子束诱变技术在小麦品质育种方面的应用提供理论依据，以Ｎ⁺ （３０Ｋｅｖ）注入诱导获得的小偃８１突变系Ｍ４代种子为材料，采用ＳＤＳＰＡＧＥ和ＡＰＡＧＥ技术对其高分子量麦谷蛋白亚基和醇溶蛋白进行系统分析。结果表明，在供试材料中，检测到３个高分子量麦谷蛋白亚基缺失系：１Ａｘ１缺失系、１Ｂｘ１４＋１Ｂｙ１５缺失系和１Ｄｘ２＋１Ｄｙ１２缺失系，出现频率由大到小依次为１Ａｘ１＞１Ｂｘ１４＋１Ｂｙ１５＞１Ｄｘ２＋１Ｄｙ１２。检测到５种醇溶蛋白变异类型，其中，１Ａｘ１缺失系有３种突变类型，１Ｂｘ１４＋１Ｂｙ１５缺失系和１Ｄｘ２＋１Ｄｙ１２缺失系各有１种突变类型，ω区变异类型最多，其次是α和γ区，β区没有发现变异类型，在５种变异类型中有一条相同的变异谱带。以上结果说明，Ｎ⁺离子束注入能有效地诱导小麦种子高分子量谷蛋白亚基和醇溶蛋白的变异，并能够在后代中稳定遗传。

英文摘要:

This experiment systematically analyzed high molecular weight subunits of glutenin and gliadin proteins in M₄ generation seeds of Xiaoyan 81 variation line by SDS PAGE and A PAGE techniques , seeds of Xiaoyan 81 were implanted with N⁺（30 Kev）ion beam. Three kinds of HMW GS deletion lines are detected：1Ax1 deletion line，1Bx14+By15 deletion line and 1Dx2+Dy12 deletion line，frequency of the above deletion lines occurrence is descending as 1Ax1＞1Bx14+By15＞1Dx2+Dy12. Five kinds of mutant phenotypes have detected in gliadin proteins , three of them in 1Ax1 deletion line, 1Bx14+By15 deletion line and 1Dx2+Dy12 deletion line have one respectively. The ω block has the most variations, next is α block and γ block, no mutant phenotype is detected in β block. There was one same band in the five kinds of mutation types. The results showed that N⁺（30 Kev）ion beam implantation can make the high molecular weight subunits of glutenin and gliadin proteins mutated effectively and can be stably inherited in the offspring.

查看全文查看/发表评论下载PDF阅读器

关闭