| 王 亮, 穆培源 ,徐红军, 庄 丽, 桑 伟,聂迎彬,崔凤娟,邹 波.新疆小麦品种Wx基因组成及其淀粉糊化特性研究[J].麦类作物学报,2010,30(6):1017 |
| 新疆小麦品种Wx基因组成及其淀粉糊化特性研究 |
| Characterization of Wx genes and Starch Pasting Properties of Xinjiang Winter and Spring Wheat Cultivars |
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| DOI：10.7606/j.issn.1009-1041.2010.06.006 |
| 中文关键词: 新疆 普通小麦；Wx基因；淀粉糊化特性 |
| 英文关键词:Xinjiang Common wheat（Triticum aestivum L.） Wx gene Starch pasting properties |
| 基金项目:兵团科技支疆计划项目（2008ZJ10）；兵团博士资金项目（05JC02）；国家自然科学基金项目（30560078）；国家科技支撑计划项目（2006BAD01A02-30）；兵团科技攻关计划项目（2006GG04）。 |
| 作者 | 单位 | | 王 亮1,2, 穆培源1,2 ,徐红军1,2, 庄 丽2,3, 桑 伟1,2,聂迎彬1,2,崔凤娟1,2,邹 波1 | （1.新疆农垦科学院作物研究所，新疆石河子 832000；2.谷物品质与遗传改良兵团重点实验室，新疆石河子 832000；3.石河子大学生命科学学院，新疆石河子 832002） |
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| 摘要点击次数: 1925 |
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| 中文摘要: |
| 为了给新疆小麦淀粉品质改良和优质面条小麦新品种选育提供理论依据，利用Wx A1、Wx B1和Wx D1位点的3个STS标记（MAG264、BDFL BRD和MAG269）对250份新疆小麦品种（包括157份冬小麦品种和93份春小麦品种）Wx基因的组成进行了鉴定，同时测试了185份冬、春小麦品种的淀粉糊化特性。结果表明，新疆小麦品种（系）Wx基因组成比较单一，以野生型（Wx A1a/Wx B1a/Wx D1a）为主，占参试品种总数的84.0%，除了在Wx B1位点发现亚基缺失的品种（缺失比例为13.6%）外，没有发现其他亚基缺失类型。Wx B1蛋白亚基缺失比例在冬、春小麦间差异较大，春小麦品种Wx B1亚基缺失比例（21.5%）要高于冬小麦品种（8.9%）；农家品种、引进品种和育成品种中Wx B1亚基缺失比例也各不相同，依次为0%、5.6%和22.6%。分析表明，新疆小麦品种淀粉糊化特性变异范围相当广泛，冬、春小麦品种在峰值黏度、稀澥值和反弹值等主要淀粉指标上均达到极显著差异，春小麦品种的淀粉特性优于冬小麦品种，并且Wx B1亚基缺失体淀粉糊化特性的平均表现总体优于非缺失体。本研究所用3个分子标记对小麦基因组DNA扩增的稳定性、重复性较好，检测结果准确、可靠，可以用于小麦品种Wx基因组成的鉴定和糯质专用小麦的分子标记辅助选育。 |
| 英文摘要: |
| In order to accelerate the progress for the improvement of wheat starch quality and provide useful information for noodle wheat quality improvement in Xinjiang, 250 wheat cultivars and advanced lines, including 157 winter wheat cultivars and 93 spring wheat cultivars, were identified for the composition of Wx genes using STS markers (MAG264、BDFL BRD and MAG269) at three loci Wx A1,Wx B1 and Wx D1. We also tested the starch pasting properties of 185 winter and spring wheat cultivars. The results indicated that the composition of Wx gene was not abundant in Xinjiang wheat cultivars, and the wild genotype Wx A1a/Wx B1a/Wx D1a was the major type in Xinjiang wheat cultivars with a percentage of 84.0%, and none of these cultivars missed two or three waxy protein subunits. The cultivars with null Wx A1 or Wx D1 were not detected. We only found 34 cultivars with Wx B1 Null (Wx B1b genotype), with a percentage of 13.6%. The percentage of cultivars with Wx B1b was significantly different between winter and spring wheat cultivars. The percentage of Wx B1b in spring wheat cultivars (21.5%) was much higher than that in winter wheat cultivars (8.9%), and the percentage of Wx B1b genotype were also different in landraces, introduced cultivars and cultivars bred in Xinjiang, and their percentages were 0%, 5.6% and 22.6%, respectively. The results showed that the genetic variation of starch pasting properties in Xinjiang wheat were much wider than that from other regions in China, and the main starch pasting parameters, including peak viscosity, breakdown and set back, showed highly significant differences at 1% probability level between winter and spring wheat cultivars, and the starch properties of spring wheat cultivars are better than that of winter wheat cultivars, and starch pasting properties of cultivars with Wx B1 Null are better than the wild cultivars. The result also indicated that the three molecular markers had characteristics of good stability, accuracy and reliability for amplifying wheat genomic DNA, thus they could be directly used for identifying Wx genes and marker assisted selection of waxy wheat cultivars. |
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