赵春艳,曾亚文,普晓英,杜 娟,杨树明,张再懿,谢勇武.大麦基因型间SSR标记的多态性及其亲缘关系分析[J].麦类作物学报,2010,30(6):1011 |
大麦基因型间SSR标记的多态性及其亲缘关系分析 |
Genetic Diversity and Genetic Relationships amongof Barley Genotypes Using SSR Markers |
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DOI:10.7606/j.issn.1009-1041.2010.06.005 |
中文关键词: 大麦 亲缘关系 微卫星标记 多样性 |
英文关键词:Barley varieties (lines) Genetic Relationships SSR maker Diversity |
基金项目:国家现代农业产业技术体系建设专项资金项目;农业部公益性农业科研专项(nyhyzx07 001 大麦);国家科技支撑计划项目(2006BAD02B04 11)。 |
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中文摘要: |
为了解大麦种质资源的遗传多样性,利用位于大麦7条染色体上的42对SSR引物对42个大麦种质多态性及其亲缘关系进行了分析,结果表明,42对SSR引物在42个大麦品种间均有多态性,共检测到112个等位基因,每一对引物的等位基因个数在2~5之间,平均为2.591个。42对SSR引物的多态性信息含量(PIC)变幅为0.321~0.857,平均为0.433。聚类结果表明,这些大麦基因型间的遗传相似系数分布范围较小,为0.321~0.857,在遗传相似系数值0.550水平上,这些品种聚成4大类。SSR标记揭示多数二棱啤酒大麦和多棱饲料大麦品种分别聚在不同类群中,表明其遗传距离较远,遗传基础较广泛;同一类品种(系)间的分布范围相对较近,表明其遗传基础较狭窄;大麦地方品种遗传背景相对复杂。 |
英文摘要: |
The genetic diversity and genetic relationships of 42 barley varieties(lines) were analyzed by 42 pairs of SSR makers. The results showed that the 42 pairs of SSR primers separated on the 7 chromosomes of barley, detected polymorphism at 112 alleles in total. The average number of alleles per SSR locus was 2.591 with a range from 2 to 5.The value of allelic polymorphism formation content(PIC) ranged from 0.321 to 0.857 with an average of 0.433 per SSR maker. UPGMA cluster analysis showed that the range of genetic similarity coefficient located between 0.321~0.857. At the similaritg coefficient of 0.550, these barley varieties(lines) could be classified into 4 groups. The majority of two rowed beer and poly rowed feed varieties (lines) were separately located in different groups, indicated that the genetic basis was rather extensive; In the same groups, the barley varieties (lines) had relatively narrow range, indicated that their genetic basis was more narrow;Relatively genetic background of barley landraces were complex. |
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