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为了明确大麦成株叶锈病抗性品种‘Pompadour’抗性基因的性质，以‘ＷＡＢＡＲ2482×Ｐｏｍｐａｄｏｕｒ’的４5７个F₂后代为材料，采用单链构象多态性（ＳＳＣＰ）分析技术，对发掘的ＳＮＰＥＳＴ分子标记在抗性品种的F₂后代中进行分子标记检测。结果表明，４5７株F₂分离群体中３２８株表现抗病，１２９株表现感病，卡方检验符合３∶１的分离比例。推断新发现的5ＨＳ成株叶锈病抗性基因为主效显性抗性基因。在所筛选的１5对大麦5ＨＳ染色体ＥＳＴ标记中，共显性ＳＮＰ标记Ｋ05820共检测出１０９株抗性亲本纯合体、１１5株感病亲本纯合体和２３３株杂合体。利用F₂代表型分离结果对标记Ｋ05820进行验证分析发现，该标记与‘Ｐｏｍｐａｄｏｕｒ’5ＨＳ染色体成株叶锈病抗性高度相关（r＝０．８２）。同时，新的定位分析表明，Ｋ05820 与成株叶锈病抗性基因间的遗传距离为７ｃＭ。这说明共显性ＥＳＴＳＮＰ标记Ｋ05820 可以运用于大麦5ＨＳ染色体成株叶锈病抗性基因后代杂合体及纯合体的筛选，能够应用于田间抗病材料的初步筛选。

英文摘要:

The research was conducted to find out a useful marker from barley 5HS EST to analyze the F₂ population derived from an adult plant leaf rust resistant variety ‘Pompadour’. For mining single nucleotide polymorphism (SNP) markers, single strand conformation polymorphism (SSCP) technique was used to distinguish heterozygous and homozygous in 457 F₂ population derived from ‘WABAR2482×Pompadour’. F₂ phenotype data demonstrated that 328 plants were found in resistant group and 129 plants were included in the susceptible group. The new gene was postulated as a major dominant gene controlling the adult plant resistance on barley 5HS by a segregation ration 3∶1. Besides, the co dominant EST SNP marker K08520 tested 109 resistant homozygous, 115 susceptible homozygous and 233 heterozygous in 457 F₂ population. Association analysis demonstrated that correlation coefficient between the marker K08520 and the adult plant resistance was 0.82, which shows that EST SNP K08520 could be used in the molecular assisted selection (MAS) for the adult plant resistance (APR) to barley leaf rust in Pompadour. In addition, the segregation data displayed that K08520 was mapped 7cM to the new APR gene. The new developed EST SNP marker K08520 is a useful molecular marker for analyzing heterozygous, homozygous and MAS for the adult plant resistance in ‘WABAR2482×Pompadour’ F₂ population.

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