| 杨恩年，唐宗祥，李 俊，杨武云，邹裕春.小麦育种亲本SW3243中1BL/1RS易位的细胞学和分子标记检测及来源分析[J].麦类作物学报,2010,30(5):796 |
| 小麦育种亲本SW3243中1BL/1RS易位的细胞学和分子标记检测及来源分析 |
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| DOI：10.7606/j.issn.1009-1041.2010.05.003 |
| 中文关键词: 小麦 SW3243 1BL/1RS Giemsa C带 特异PCR |
| 英文关键词:SW3243 1BL/1RS Giemsa C banding Specific PCR |
| 基金项目:科技部国际合作项目四川 CIMMYT合作选育条锈病微效多基因持久抗性小麦新品种（系）（2007DFA31480）。 |
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| 中文摘要: |
| 为了深入了解小麦育种亲本SW3243的遗传基础，利用多重PCR引物对SW3243及其他47个材料进行1BL/1RS 易位系检测，同时对SW3243进行Giemsa C带分析，并利用5对定位于黑麦染色体1RS上的特异PCR引物对1BL/1RS易位系进行了多样性分析。多重PCR分析结果表明，包括SW3243在内共23个材料含有1BL/1RS易位。细胞学研究结果证实，SW3243含有1对1BL/1RS 易位染色体；筛选到定位于黑麦1RS上的3对SSR引物（Xmwg913、Xmwg2062a、SCM9）可用于区别不同的1BL/1RS易位染色体；用筛选到的3对特异PCR引物对SW3243及其他22个1BL/1RS品种（系）进行分析，结果表明SW3243所含1BL/1RS 易位染色体与SW22514、西科麦2号、山前麦、川麦32、川麦35的1 BL/1 RS易位染色体不同。 |
| 英文摘要: |
| The plant area of SW3243 and its derived varieties were totally extended to 4.33 million hectare in Sichuan, and 7 varieties derived from SW3243 were released in Sichuan province and 3 were released in national level. Giemsa C banding displayed that dwarf, large spike and high yielding wheat breeding parent SW3243 had a pair of chromosomes 1RS/ 1BL. Moreover, 3 Specific PCR primers were selected to test SW3243 and other 22 varieties, Specific PCR testing indicated that SW3243 contained 1RS/ 1BL, but its 1RS/ 1BL was different from these 1RS/ 1BL contained by varieties SW22514, Xikemai 2, Predgornia, Chuanmai 32 and Chuanmai 35. |
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