陆宁海,王建锋, 詹刚明, 黄丽丽,康振生.小麦条锈菌毒性变异与DNA多态性的相关性[J].麦类作物学报,2010,30(3):568 |
小麦条锈菌毒性变异与DNA多态性的相关性 |
Relationship between Virulence Variation and DNA Polymorphism in Puccinia striiformis f. sp. tritici |
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DOI:10.7606/j.issn.1009-1041.2010.03.036 |
中文关键词: 小麦 条锈菌 毒性 DNA多态性 相关性 |
英文关键词:Wheat Puccinia striiformis f. sp. tritici Virulence DNA polymorphism Relativity |
基金项目:国家“973”项目(2006CB100203);现代农业产业技术体系建设专项;国家“十一五”支撑计划项目(2006BAD08A05);教育部长江学者和创新团队发展计划资助项目(200558);教育部“111”引智项目(B07049) 。 |
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中文摘要: |
为了解小麦条锈菌毒性和DNA多态性之间的相关性,为SSR标记技术在条锈菌群体遗传分析中的应用提供理论依据,对90个小麦条锈菌菌系进行了毒性和DNA多态性分析。毒性分析发现17种致病类型或生理小种, SSR分子标记分析发现75个基因型,DNA指纹的多态性远比毒性的多态性丰富。不同小种有相同或相近的DNA指纹,同一小种可能有不同的DNA指纹。毒性特征和DNA多态性之间并不存在相关性,SSR标记技术可用于小麦条锈菌群体遗传分析。 |
英文摘要: |
To determine the relationship between virulence and DNA polymorphism, and to evaluate usability of SSR marker in detecting population genetic diversity of Puccinia striiformis f. sp. tritici, relationship between virulence variation and DNA polymorphism of 90 isolates of Puccinia striiformis f. sp. tritici were studied. 17 pathotypes and 75 genotypes were found, suggesting DNA polymorphism was much more diversity than virulence. Moreover, there were the same or near genotype in different races, and the same race may be had different genotype. There was no relativity between virulence and DNA polymorphism. SSR markers are considered appropriate for population genetic diversity analysis. |
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