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作者	单位
薛芳，褚洪雷，胡志伟，王雪梅，李卫华	(石河子大学/新疆兵团绿洲生态农业重点实验室，新疆石河子 832003)

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中文摘要:

为给甲基磺酸乙酯（EMS）在高抗性淀粉小麦诱变育种中的应用提供依据，以春小麦新春11为材料，分析了不同浓度EMS诱变小麦种子的M₂代成熟籽粒抗性淀粉含量的变化，同时结合株高等农艺性状对优异材料进行了筛选。结果表明，不同浓度EMS(0.3%、0.5%和0.7%)处理后，M₂代籽粒平均抗性淀粉含量分别为2.76%、3.22%和4.31%，高于未处理的籽粒抗性淀粉含量（2.57%），并发现了抗性淀粉含量较高和较低的突变体。其中，0.3%浓度EMS最适宜新春11农艺性状的诱变，突变类型最多；0.7%的EMS处理获得的高抗性淀粉含量的突变体更为丰富。综合农艺性状、产量性状和品质的表现，筛选出7个抗性淀粉含量高且综合性状优良的M₂突变家系。

英文摘要:

To detect the matation effects on resistant starch (RS) content. A simple and fast method of RS testing was established according to the method of AOAC2002 and used to determine the RS content of the M₂ generation seeds of Xinchun 11 treated by different concentrations of EMS(0.3%, 0.5%, 0.7%). The average RS content was 2.76 %, 3.22 %, 4.31% respectively in M₂ from treatments with different concentrations of EMS, and the RS content in untreated seeds was 2.57%. The results suggested that the RS content of M₂ mutations treated by EMS were higher than that of untreated. At the same time, the treatment by 0.3% EMS was the most suitable concentration to achieve the most of agronomic mutation individuals. But, the higher and more abundant RS content of M₂ mutations were observed in the 0.7% EMS treatment. Total 7 M₂ mutations with higher RS content and good agronomic traits were selected in this study.

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