| In order to investigate the distribution of allelic variations for genes Psy A1 and Psy B1 controlling wheat yellow pigment content on chromosomes 7A and 7B in Shaanxi wheat cultivars (lines). Functional molecular markers YP7A, YP7A 2, YP7B 1, YP7B 2 and YP7B 3 were used to detect 176 wheat cultivars (lines) released in Shaanxi province. The results showed that two allelic variations at Psy A1 locus were found in Shaanxi wheat cultivars (lines), Psy A1a and Psy A1b alleles with high and low YP content, respectively. The frequencies of both Psy A1a and Psy A1b alleles were 50.0%, and Psy A1c allele was absent in Shaanxi wheats. Three alleles Psy B1a, Psy B1b and Psy B1c at Psy B1 locus were found in Shaanxi wheat cultivars (lines). The frequencies of alleles Psy B1b, Psy B1a and Psy B1c were 65.3%, 27.3% and 7.4%, respectively. But Psy B1d allele was absent in Shaanxi wheats. Mean frequencies among allelic combinations in Shaanxi wheats were also different. The frequencies of allelic combinations Psy A1b/Psy B1b, Psy A1a/PsyB1b, Psy A1a/Psy B1a, Psy A1b/Psy B1a, Psy A1a/Psy B1c and Psy A1b/Psy B1c were 39.2%, 26.1%, 17.6%, 9.7%, 6.3% and 1.1%, respectively. The frequencies of those genotypes were also different among wheat cultivars (lines) from different regions of Shaanxi. Generally, number of cultivars with low YP content in Shaanxi province was slightly higher. This would be conducive to the selection of lower YP content cultivars in wheat quality breeding. The distribution of genotypes for YP content in different wheat regions of Shaanxi province was largely determined by their breeding target and planting requirement. The results showed that these functional markers used in this study could accurately identify the alleles of genes Psy A1 and Psy B1. They could be used as an effective tool for marker assisted selection (MAS) in wheat breeding programs targeting for either lower or higher YP content. |
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