| 刘红占，张改生，兰红玉，张明珠，李 莉，高春保，袁 蕾，胡俊敏，位 芳，牛 娜，马守才，李红霞.杂交小麦强优势组合抗白粉病基因的定向导入与改良研究初报[J].麦类作物学报,2010,30(3):415 |
| 杂交小麦强优势组合抗白粉病基因的定向导入与改良研究初报 |
| Preliminary Study on Directional Introgression and Improvement of Resistance Genes to Powdery Mildew in Hybrid Wheat |
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| DOI：10.7606/j.issn.1009-1041.2010.03.004 |
| 中文关键词: 杂交小麦 白粉病 抗病性 |
| 英文关键词:Hybrid wheat Powdery mildew (Erysiphe graminis f. sp. tritici) Disease resistance |
| 基金项目:国家高技术研究发展计划(“863”计划)重大专项(2009AA101102)；高等学校博士点基金项目（20090204110024）；陕西省“13115”科技创新工程重大科技专项（2007ZDKG 020）；国家杨凌农业生物技术育种中心专项基金项目(99 1A)；西北农林科技大学拔尖人才支持计划项目。 |
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| 中文摘要: |
| 为创制杂交小麦白粉病抗性定向改良育种新体系，以白粉病抗源材料N95175、N9134为抗病种质，以强优势杂交小麦新品种“西杂一号”和“西杂五号”的亲本为受体，以陕西不同地区小麦白粉病流行混合菌种为菌源，对供试材料亲本、杂种F1代和回交后代材料进行了苗期和成株期抗性鉴定，并利用与抗白粉病基因Pm21共分离的SCAR标记、抗白粉病基因PmAS846紧密连锁的SSR标记作为抗病性定向选择分子标记进行了抗病性鉴定。结果表明，以N95175、N9134为亲本的杂种F1含有Pm21基因或PmAS846基因，苗期和成株期均表现为高抗，与抗病亲本的抗病性表现基本一致，符合杂种优势表现显性假说，其抗病性主要由显性单基因控制。研究同时表明，仅凭借表型鉴定结果盲目性较大，表型鉴定和分子标记鉴定相结合准确率较高。 |
| 英文摘要: |
| To develop a combination of wheat hybrid system with high resistance to powdery mildew diseases, we used N9134, N95175 as sources for resistance to powdery mildew to improve the parents of the strong advantage of series of ‘XIZA’ hybrid varieties of wheat. Wheat powdery mildew fungus strains from different regions in shaanxi province were collected and used to inoculate the parents, F1 offspring and backcross offspring. Meanwhile, the SCAR markers co separated with Pm21 gene and the SSR marker closely linked with PmAS846 gene were used to identification of resistance. The results showed that F1 hybrids derived from N95175, N9134 with Pm21 or PmAS846 were characterized with high resistance at seedling and adult plant period, and they possessed identical disease resistance as resistant parent, which accorded with the heterosis dominance hypothesis. Besides, plants showing disease resistance did not produce molecular markers, which indicated an inaccuracy of molecular markers present sometimes. We established an improved hybrid wheat breeding system with high disease resistance, and therefore succeeded to maintain the original heterosis of wheat hybrids as well as other agronomic traits, which provided a novel avenue for super hybrid wheat breeding. |
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