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作者	单位
李慧1 ，赵文才1 ，赵会杰2 ，韩燕来1，谭金芳1	1. 河南农业大学资源与环境学院，河南郑州 450002； 2. 河南农业大学生命科学学院，河南郑州 450002

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中文摘要:

为了研究外源物质对小麦抗旱性的诱导作用，水培条件下研究了外源腐胺对干旱胁迫(15%PEG 6000）条件下冬小麦幼苗叶片膜脂过氧化及抗氧化酶活性的影响。结果表明，干旱胁迫48 h后冬小麦幼苗叶片含水量下降了27.43%， O2的产生速率、丙二醛(MDA)和H2O2含量随时间的推移均呈增加的趋势，SOD、POD、CAT活性分别下降了23%、37%、70%。外源腐胺处理下叶片含水量仅下降了7.62%；叶片 MDA含量降低80%左右，H2O2的含量下降50％左右，O2的产生速率降低了1/3，而叶片SOD、POD、CAT活性分别提高了41%、165.8%、246%。因此认为，外源腐胺可通过抑制活性氧（O2，H2O2）的产生，降低MDA含量，提高抗氧化酶（SOD、POD、CAT）的活性，缓解干旱胁迫造成的冬小麦幼苗叶片细胞膜脂过氧化损伤，提高冬小麦的抗旱性。

英文摘要:

In the nutrient solution culture conditions, the effects of exogenous putrescine on membrane lipid peroxidation and antioxidant enzyme activity in wheat seedling leaves were investigated under 15% PEG 6000 drought stress. The results indicated that at 48 h after drought stress, content of water in wheat seedling leaves decreased by 27.43%, superoxide (O2) production rate, MDA and H2O2 content increased, while the SOD, POD, CAT activities decreased by 23%, 37%, 70%, respectively. Compared with drought stress, content of water in wheat seedling leaves only decreased by 7.62% in the treatment of 100 μmol·L-1 putrescine, accumulation of MDA declined about 80%, content of H2O2 declined about 50%，and O2 production rate reduced by 35%; however, SOD、CAT and POD activities increased by 41%, 165.8%, 246% in winter wheat seedling leaves. It indicated that exogenous putrescine alleviate membrane oxidative damage in winter wheat seedlings possible by inhibiting reactive oxygen species (O2, H2O2) production, decreasing content of MDA and enhancing the antioxidant enzyme (SOD, POD, CAT) activity, to improve drought resistance of winter wheat.

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