| 庄 静1,3,陈建民2,彭日荷1,高 峰1,朱 波1,
付晓燕1,ZHANG Jian3,熊爱生1,姚泉洪1.一个小麦AP2/ERF转录因子家族单独亚族基因的克隆及分析[J].麦类作物学报,2009,29(5):752 |
| 一个小麦AP2/ERF转录因子家族单独亚族基因的克隆及分析 |
| Cloning and Analysis of an AP2/ERF Soloist Subfamily Transcription Factor from Triticum aestivum L. Yangmai 15 |
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| DOI：10.7606/j.issn.1009-1041.2009.05.003 |
| 中文关键词: 小麦 AP2/ERF转录因子 基因克隆 |
| 英文关键词:Triticum aestivum L. AP2/ERF Transcription factor Gene clone |
| 基金项目:国家“863”项目(2006AA10Z117)； 上海市青年科技启明星跟踪计划项目(08QH14021), 上海市自然科学基金项目（08ZR1417200）；上海市-Alberta国际合作项目(08540706500)；国家转基因重大专项(2009ZX08002 011B)。 |
| 作者 | 单位 | | 庄 静1,3,陈建民2,彭日荷1,高 峰1,朱 波1,
付晓燕1,ZHANG Jian3,熊爱生1,姚泉洪1 | 1.上海市农业科学院，上海 201106
2. 扬州大学生物科学与技术学院，江苏扬州 225009
3 . 加拿大艾伯塔省研究院, 埃德蒙顿 T9C1T4 |
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| 摘要点击次数: 1888 |
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| 中文摘要: |
| AP2/ERF家族转录因子是植物中重要的一类转录因子，广泛参与植物各类生理过程。为了给该家族转录因子的深入研究提供基础，利用小麦EST数据库(http://www.ncbi.nlm.nih.gov/UniGene/UGOrg.cgi?TAXID= 4565)，以拟南芥AP2/ERF家族转录因子单独亚族成员AtAP2 Soloist的氨基酸序列为探针，搜索到一个小麦UniGene数据，经拼接得到1个全长cDNA序列（TaAP2 Solosit），通过RT PCR方法从“扬麦15”的cDNA中克隆了该转录因子基因（YM15AP2 Solosit）。克隆的转录因子核苷酸序列与电子克隆的序列只有1个碱基不同，两者编码氨基酸序列一致。从cDNA序列、氨基酸序列的相似性、组成成分、理化性质、疏水性/亲水性、进化树、功能域、二级和三级结构等方面进行了分析，结果显示“扬麦15”中的YM15AP2 Solosit转录因子属于AP2/ERF家族中的单独亚族，是亲水性蛋白，在蛋白质的三级结构上与拟南芥的AtAP2 Soloist相似。EST丰度分析显示，YM15AP2 Solosit在根、茎、叶和花中均有表达。 |
| 英文摘要: |
| AP2/ERF is a large family of plant transcription factors. Genes in the AP2/ERF family encode transcriptional regulators that serve a variety of functions in the plants. An AP2/ERF family transcription factors (TaAP2 Solosit) was isolated by silico cloning method using the AtAP2 Soloist amino acid sequence of Arabidopsis thaliana as probe from wheat (Triticum aestivum L.) ESTs (http://www.ncbi.nlm.nih.gov/UniGene/UGOrg.cgi?TAXID=4565). Based on the sequences of TaAP2 Solosit, YM15AP2 Solosit gene was isolated from wheat Yangmai 15 by RT PCR using cDNA as template. Only one oligonucleotide was different between TaAP2 Solosi and YM15AP2 Solosit but their amino acid sequences were the same. Then, cDNA and deduced amino acid sequence, composition, hydrophobicty and hydrophilicity, physical and chemical characterization, phylogenetic tree, conserved domain sequences, function domain and molecular modeling were analyzed. YM15AP2 Solosit was belonged to Solosit subfamily of AP2/ERF family. YM15AP2 Solosit was hydrophilic protein. TaAP2 Solosi and YM15AP2 Solosit have similar three dimension structure. YM15AP2 Solosit was expressed in seed, stem, leaf and inflorescence. |
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