| 陈 龙1 ，侯彩玲2， 谭光轩1，于美玲2，卢龙斗2 ，殷贵鸿3.小麦糯性基因多重PCR体系的建立[J].麦类作物学报,2009,29(5):747 |
| 小麦糯性基因多重PCR体系的建立 |
| Establishment of Multiplex PCR for Waxy Genes in Wheat |
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| DOI：10.7606/j.issn.1009-1041.2009.05.002 |
| 中文关键词: 小麦 糯性基因 多重PCR |
| 英文关键词:Wheat Waxy gene Multiplex PCR |
| 基金项目:河南省重点科技攻关项目（072102120011）。 |
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| 中文摘要: |
| 为建立通过一次反应能够同时鉴定3个小麦糯性基因（Wx A1、Wx B1和Wx D1）的多重PCR反应体系，研究了PCR反应组分和循环参数对多重PCR反应结果的影响。结果表明，反应体系中的引物浓度比例和反应的Tm值是多重PCR成功的关键，当引物浓度比例（SSR/MAG267）为1（0.1 μmol/L∶0.1 μmol/L）~1.22（0.11 μmol/L∶0.09 μmol/L）、Tm为58℃时，Wx A1、Wx B1、Wx D1基因的多重PCR结果最好。该多重PCR方法能快速高效地鉴定3个Wx基因，可用于糯麦选育和小麦品质育种的复合分子标记辅助选择。 |
| 英文摘要: |
| The research studied the influences of PCR components and cyclic parameters on the multiplex PCR and established a multiplex PCR system to simultaneously identify Wx A1、Wx B1 and Wx D1 genes of wheat in one reaction. The result showed that the primers concentration ratio and Tm value were the critical factors to influence effectiveness of multiplex PCR, when the primers concentration（SSR/MAG267）was 1（0.1 μmol/L∶0.1 μmol/L）~1.22（0.11 μmol/L∶0.09 μmol/L）and Tm=58℃, the outcome of multiplex PCR of Wx A1, Wx B1 and Wx D1 was the best. The multiplex PCR system identifying three Wx genes quickly and efficiently simultaneously in one reaction can be used to carry out multiplex molecular marker assisted selection in breeding waxy wheat cultivars and quality wheat breeding. |
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