| 杨 涛1，段承俐1，3，杨生超1，文国松1 ，曾亚文2，萧凤回1，3.大麦籽粒中4种主要黄酮类化合物的HPLC测定方法研究[J].麦类作物学报,2009,29(4):618 |
| 大麦籽粒中4种主要黄酮类化合物的HPLC测定方法研究 |
| HPLC Determination of Catechin, Myricetin, Quercetin and Kaempferol in Barley Grain |
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| DOI：10.7606/j.issn.1009-1041.2009.04.014 |
| 中文关键词: 大麦 儿茶素 杨梅素 槲皮素 山奈酚 高效液相色谱 |
| 英文关键词:Barley Catechin Myricetin Quercetin Kaempferol HPLC |
| 基金项目:云南省中药现代化科技专项（2002ZY 24）；农业部公益性行业科研专项（nyhyzx07 001 大麦)；农业部“948”重大项目(2006 G9 5)；国家科技支撑计划项目(2006BAD02B04)。 |
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| 中文摘要: |
| 为给大麦主要黄酮类化合物含量的快速测定及开发利用提供依据，对利用高效液相色谱技术（HPLC）分离和同时测定大麦中黄酮类化合物儿茶素、杨梅素、槲皮素、山奈酚含量的方法进行了研究。结果表明，采用HPLC同时测定4种大麦黄酮类化合物的优化色谱条件为：YMC Pack ODS AM 303 (5 μm, 250 mm_4.6 mm i.d.)色谱柱；流动相A 0.1%冰乙酸水溶液，B 乙睛，梯度洗脱；流速0.8 mL/min，进样量10 μL。儿茶素、杨梅素、槲皮素、山奈酚分别在0.063～2.000 μg (R2=0.9999)、0.034~1.100 μg (R2=0.9998)、0.025~0.800 μg (R2=0.9993)、0.018~0.560 μg (R2=0.9995)成良好的线性关系，加样回收率分别为96.88%(RSD=1.30%)、98.30%(RSD=0.57%)、96.29%(RSD=1.20%)、101.59%(RSD=0.73%)。 测定方法简便、快捷，结果准确、重复性好，可用于大麦4种主要黄酮类化合物的同时测定。 |
| 英文摘要: |
| In order to determine the contents of barley flavonoids of catechin, myricetin, quercetin and kaempferol, the use of HPLC Separation and Determination of Flavonoids in Barley catechin, myricetin, quercetin, kaempferol content were investigated. The results showed that while the HPLC was carried out on MC Pack ODS AM 303 (5 μm, 250 mm_4.6 mm i.d.) column, the mobile phase was distilled water with 0.1% glacial acetic acid (solvent A) and acetonitrile (solvent B), the flow rate was 0.8 mL·min-1; and 10 μL sample of each filtrate was used, the calibration curves were linear in the range of 0.063~2.000 μg (R2=0.9999) for catechin, 0.034~1.100 μg (R2=0.9998) for myricetin, 0.025~0.800 μg (R2=0.9993) for quercetin and 0.018~0.560 μg (R2=0.9995) for kaempferol, with average recoveries of 96.88%(RSD=1.30%), 98.30%(RSD=0.57%), 96.29%(RSD=1.20%) and 101.59%(RSD=0.73%), respectively. The assay demonstrated that the method was simple, accurate to quantify the four active components in barley simuniously. |
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