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作者	单位
徐红明^1,2，刘红彦²，王俊美²，田保明¹，王鹏涛²	（1. 郑州大学生物工程系，河南郑州 450001； 2. 河南省农业科学院植物保护研究所，河南郑州 450002）

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中文摘要:

为了研究Mlo基因在感、抗小麦品种中的表达情况，根据小麦基因芯片的Mlo探针序列，利用电子克隆与RT PCR方法在感病小麦品种豫麦13和抗病品种红蚰麦中分别克隆到Mlo基因的同源序列。两条核苷酸序列全长均为1 605 bp，都含有一个完整的ORF(Open reading frame)。核苷酸序列分析显示二者的DNA序列只有一个核苷酸的差异，与大麦Mlo的相似性均为90%。编码的氨基酸序列包含有7个跨膜结构域，前18个氨基酸是信号肽序列，第414~435位是编码蛋白的活性中心，第451、459、462、475、483位点上有糖基化位点，细胞定位分析将其定位在膜上。从头建模分析构建的二者三维结构有明显差异。利用定量PCR对该基因在白粉菌诱导早期的表达模式进行分析，结果两基因的表达都受白粉菌的诱导，但在红蚰麦中的表达高峰出现在18 h，豫麦13中的表达高峰出现在72 h，表达时间的差异可能决定了两者对白粉菌的不同抗性反应。

英文摘要:

To investigate the expression mode of Mlo gene in susceptible and resistant wheat,two homological sequences of Mlo gene were cloned from susceptible cultivar Yumai 13 and resistant Hongyoumai by in silico cloning and RT PCR in this study, according to Mlo probe sequence of the wheat gene chip. Two obtained sequences differed in one nucleotide acid and both contained an integrated open reading frame of 1 605 bp length with 90% identical nucleotide with Mlo from baley. Amino acid analysis indicated that the encoded aa（amino acid）contained 7 transmembrane domains and original 18 amino acids were signal peptide. The aa during 414~435 was active site and 451, 459, 462, 475 and 483 aa were glycosylated. Mlo was located on cell membrane. Difference in the three dimensional structure was prominent between Mlo Yumai13 and Hongyoumai by ab initio prediction. Their expression pattern was analyzed by quantitative PCR, results demonstrated that expression of both genes were induced by Egt（Erysiphe graminis f. sp. tritici）, the expression peak of Hongyoumai appeared in the time point of 18 h, while, 72 h of Yumai 13. The difference of expression peak may determine different resistant responses to Egt.

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