In order to explore the effects of different methods of actinomycetes on the growth and physiological characteristics of late-sown winter wheat, the experiment was carried out in the field adopting five treatments, taking Chang 6990 as the test variety, and the Streptomyces roche (D74) as the test microbial agent. The five treatments, respectively as T1(seed coating plus spraying D74 at jointing and filling stage) , T2(seed coating plus spraying D74 at jointing stage and clean water at filling stage), T3(no seed coating plus spraying D74 at jointing and filling stage), T4(seed coating plus spraying clean water at jointing and filling stage) and CK(no seed coating plus spraying clean water at jointing and filling stage), were studied on the effects of yield and its components, phenotypic traits, photosynthetic characteristics and dry matter accumulation of Chang 6990. The results showed that the yield of late-sown wheat increased in different degrees by 7.35%~25% after using actinomycetes, and the order of yield was T4>T1>T2>T3>CK, among this the yield of T1 and T4 were significantly(P<0.05) higher than CK. There was no significant difference in plant height among different treatments. The spike length of CK was significantly (P<0.05) lower than those of T1 and T4, and the grains per spike and 1000-grain weight of CK were significantly (P<0.05) lower than those of T1, T2 and T4. The effects of using actinomycetes on the photosynthetic efficiency of late-sown wheat were mainly in the late filling stage, during the 28d~35d after anthesis, the net photosynthetic rate of flag leaf of T1 and T4, the relative content of chlorophyll (SPAD) and nitrogen content of flag leaf of T1, T2, T3 and T4, and the leaf area index (LAI) of T1, T2 and T4 were significantly(P<0.05) higher than those of CK. The dry matter translocation amount before anthesis(DMTAA) and the contribution rate of dry matter translocation amount before anthesis to grains(CDMTAATG) of late-sown wheat after using actinomycetes decreased, but the difference among treatments was not significant; The dry matter accumulation amount after anthesis(DMAAA) in T1, T2, T4 increased significantly(P<0.05) by 37.93%, 29.42%, 50.92% compared with CK; The Contribution of dry matter assimilation amount after anthesis to grain(CDMAAAG) also increased, but there was no significant difference among treatments. The above results showed that the use of actinomycetes could effectively increase the photosynthetic sustainability of late-sown wheat, delay leaf senescence, improve dry matter accumulation after anthesis, promote ear development and grain filling, increase the grains per spike and 1000-grain weight, and then increase the yield. Among them, spraying actinomycetes on leaves at jointing and filling stage had the best effect. |